Monocytes from Pregnant Women with Pre-Eclampsia are Polarized to a M1 Phenotype
Monocytes from Pregnant Women with Pre-Eclampsia are Polarized to a M1 Phenotype
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DOI：
10.1111/aji.12222
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发表时间：
2014-07-01
影响因子：
3.6
通讯作者：
Peracoli, Maria T. S.
中科院分区：
文献类型：
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作者：
Medeiros, Leonardo T. L.;Peracoli, Jose C.;Peracoli, Maria T. S.
问题 本研究评估了先兆子痫 (PE) 中的单核细胞炎症状态是否可能与极化为经典 M1 或 M2 替代激活的单核细胞亚群相关。研究方法 85 名患有 (PE) 的孕妇和 52 名与胎龄匹配的血压正常 (NT) 孕妇被纳入研究。通过流式细胞术评估外周血单核细胞中M1特征性表面受体的表达，例如Toll样受体(TLR)2、TLR4和CD64，或M2，例如CD163和CD206单核细胞亚群。通过 ELISA 评估单核细胞培养物上清液中的肿瘤坏死因子-α (TNF-)、白介素-(IL)-12p40、IL-12p70 和 IL-10。结果 与NT孕妇相比，子痫前期女性单核细胞TLR4和CD64的表达显着升高，而CD163和CD206的表达显着降低。 PE 孕妇的单核细胞内源性 TNF-、IL-12p40 和 IL-12p70 的产生增加，而 IL-10 的合成低于 NT 孕妇。结论 PE 女性的单核细胞典型地被激活，产生更高水平的促炎细胞因子，并表达 M1 亚群特征的表面受体。这些结果提供证据表明，PE 中的全身炎症环境可能使这些细胞分化并极化为 M1 表型。
Problem This study evaluated whether the monocyte inflammatory state in pre-eclampsia (PE) might be associated with polarization to either M1 classically or M2 alternatively activated monocyte subsets. Method of Study Eighty-five women with (PE) and 52 normotensive (NT) pregnant women matched for gestational age were included. Expression of surface receptors characteristic of M1, such as Toll-like receptor (TLR)2, TLR4, and CD64, or M2, such as CD163 and CD206 monocyte subsets were evaluated in peripheral blood monocytes by flow cytometry. Tumour necrosis factor-alpha (TNF-), interleukin-(IL)-12p40, IL-12p70, and IL-10 were evaluated in the supernatant of monocyte cultures by ELISA. Results Expression of TLR4 and CD64 by monocytes from pre-eclamptic women was significantly higher, while the expression of CD163 and CD206 expression was significantly lower compared with NT pregnant women. Endogenous production of TNF-, IL-12p40, and IL-12p70 by monocytes was increased, while synthesis of IL-10 was lower in women with PE than in NT pregnant women. Conclusions Monocytes from women with PE are classically activated, producing higher levels of pro-inflammatory cytokines, and express surface receptors characteristic of the M1 subset. These results provide evidence that the systemic inflammatory environment in PE may differentiate and polarize these cells to the M1 phenotype.