Identification of mouse YB1/p50 as a component of the FMRP-associated mRNP particle
Identification of mouse YB1/p50 as a component of the FMRP-associated mRNP particle
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DOI：
10.1006/bbrc.2000.4035
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发表时间：
2000-12-29
影响因子：
3.1
通讯作者：
Warren, ST
中科院分区：
文献类型：
--
作者：
Ceman, S;Nelson, R;Warren, ST
脆性X智力迟钝是由于缺乏FMRP引起的，FMRP是一种rna结合蛋白，存在于一个大的mRNP复合体中。尽管有证据表明FMRP作为一种同源多聚体存在，但在mRNP中已经发现了与FMRP相关的其他蛋白质。FMRP的常染色体类似物FXR1P和FXR2P与FMRP相关，核仁蛋白和NUFIP1也与FMRP相关，它们都是RNA结合蛋白。使用稳定转染Flag-Fmr1的细胞系，我们发现了一种与FMRP共免疫沉淀的额外蛋白质。该蛋白约50 kDa，经质谱鉴定为小鼠Y盒结合蛋白1 (YB1)，与核心mRNP蛋白p50（一种rna结合蛋白）有97%的同源性。抗p50抗血清识别出50ktpa蛋白，证实了该鉴定。FMRP- mrnp与Y盒蛋白（后者通常在mrnp中发现）的关联进一步表明FMRP参与翻译调节。(C) 2000年学术出版社。
Fragile X mental retardation is caused by the absence of FMRP, an RNA-binding protein found in a large mRNP complex. Although there is evidence that FMRP exists as a homo-multimer, additional proteins have been identified that associate with FMRP in the mRNP. The autosomal paralogs of FMRP, FXR1P, and FXR2P, associate with FMRP, as do nucleolin and NUFIP1, all RNA binding proteins. Using cell lines that were stably transfected with Flag-Fmr1, we identified an additional protein that coimmunoprecipitates with FMRP. The approximately 50 kDa protein was identified by mass spectrometry as mouse Y box-binding protein 1 (YB1), which is 97% identical to the core mRNP protein p50, an RNA-binding protein. An anti-p50 antiserum recognized the 50 kTPa protein, confirming the identification. The association of the FMRP-mRNP with a Y box protein, the latter commonly found in mRNPs, further suggests the involvement of FMRP in translation modulation. (C) 2000 Academic Press.