Characterization of Treponema pallidum Dissemination in C57BL/6 Mice.
Characterization of Treponema pallidum Dissemination in C57BL/6 Mice.
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DOI：
10.3389/fimmu.2020.577129
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发表时间：
2020
影响因子：
7.3
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Lu S;Zheng K;Wang J;Xu M;Xie Y;Yuan S;Wang C;Wu Y
螺旋体病原体梅毒螺旋体每年在全世界造成500万新的性病梅毒病例。梅毒预防和治疗的一个主要障碍是缺乏合适的实验动物模型来研究其发病机制。因此，在本研究中，我们进一步评估了小鼠对梅毒螺旋体的反应。定量聚合酶链反应显示，梅毒螺旋体可在C57 BL/6小鼠的心脏、肝脏、脾脏、肾脏和睾丸中定植，感染后24 h可迅速穿透血脑屏障。在随后的家兔感染性试验中，我们在小鼠淋巴结悬液中观察到明显的微生物迹象。感染后，C57 BL/6小鼠组织中的细菌负荷高于血液中的细菌负荷。此外，通过细胞因子测定记录了显著的Th 1免疫应答。流式细胞仪检测显示，感染小鼠脾细胞中CD 3 + T细胞和CD 4 + T细胞比例明显增加。因此，提高对C57 BL/6小鼠对梅毒螺旋体反应的认识，将有助于全面阐明该菌的致病机制，并为建立新型的梅毒螺旋体研究模型奠定基础。
The spirochetal pathogen Treponema pallidum causes 5 million new cases of venereal syphilis worldwide each year. One major obstacle to syphilis prevention and treatment is the lack of suitable experimental animal models to study its pathogenesis. Accordingly, in this study, we further evaluated the responses of mice to Treponema pallidum. Quantitative polymerase chain reaction showed that Treponema pallidum could colonize the heart, liver, spleen, kidneys, and testicles of C57BL/6 mice, and the organism may be able to rapidly penetrate the blood-brain barrier in mice by 24 h after infection. In subsequent rabbit infectivity tests, we observed evident signs of the microorganism in the mouse lymph node suspension. After infection, bacterial loads were higher in the tissues than in the blood of C57BL/6 mice. Moreover, a significant Th1 immune response was recorded by cytokine assays. Flow cytometric analysis suggested an obvious increase in the proportion of CD3+ T and CD4+ T cells in the spleen cells in the infected mice. Thus, improving our understanding of the response of C57BL/6 mice for Treponema pallidum will help to comprehensive elucidate the pathogenic mechanisms of this bacterium and lay the foundation for the development of a new research model of Treponema pallidum.
东北地区伯氏疏螺旋体SZ株的病原学分析
影响因子：
3.2
作者：
Wu Q;Liu Z;Wang J;Li Y;Guan G;Yang J;Chen Z;Luo J;Yin H
通讯作者：
Yin H