Manganic porphyrins possess catalase activity and protect endothelial cells against hydrogen peroxide-mediated injury
Manganic porphyrins possess catalase activity and protect endothelial cells against hydrogen peroxide-mediated injury
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DOI：
10.1006/abbi.1997.0341
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发表时间：
1997-11-15
影响因子：
3.9
通讯作者：
Crapo, JD
中科院分区：
文献类型：
--
作者：
Day, BJ;Fridovich, I;Crapo, JD
锰卟啉是氧化还原活性金属配合物，已被用作超氧化物歧化酶模拟物。我们测试了这些金属卟啉是否也可以分解过氧化氢（H2O2），以及它们是否可以保护内皮细胞免受H2O2的侵害。这两个锰金属卟啉测试被发现催化dismute H2O2。这些锰卟啉还以剂量依赖性方式保护内皮细胞免受H2O2介导的损伤，MnTMPyP的EC 50为8 μ M，MnTBAP的EC 50为15 μ M。这些卟啉的含锌类似物在歧化H2O中是无活性的，并且不保护。这些研究进一步确定了金属卟啉在将超氧化物转化为H2O2和将H2O2转化为水方面的抗氧化能力。这些数据表明，锰卟啉可能是有用的治疗剂，对疾病状态与过度生产的活性氧。(C)北京：科学出版社.
Manganic porphyrins are redox active metal complexes that have been employed as superoxide dismutase mimics. We tested whether these metalloporphyrins could also dismute hydrogen peroxide (H2O2) and whether they could protect endothelial cells against H2O2. Both of the manganic metalloporphyrins tested were found to catalytically dismute H2O2. These manganic porphyrins also protected endothelial cells in dose-dependent manners against H2O2-mediated injury with MnTMPyP having an EC50 of 8 mu M and MnTBAP having an EC50 of 15 mu M. The zinc containing analogs of these porphyrins were inactive in dismuting H2O, and did not protect. These studies further define the antioxidant capacity of metalloporphyrins in converting superoxide to H2O2 and H2O2 to water. These data suggest that manganic porphyrins may be useful therapeutics against disease states associated with the overproduction of reactive oxygen species. (C) 1997 Academic Press.