Menthol induces cell-cycle arrest in PC-3 cells by down-regulating G2/M genes, including polo-like kinase 1
Menthol induces cell-cycle arrest in PC-3 cells by down-regulating G2/M genes, including polo-like kinase 1
复制标题
DOI：
10.1016/j.bbrc.2012.05.010
复制
发表时间：
2012-06-08
影响因子：
3.1
通讯作者：
Jeon, Ju-Hong
中科院分区：
文献类型：
--
作者：
Kim, Su-Hwa;Lee, Sanghoon;Jeon, Ju-Hong
薄荷醇是一种天然存在的单萜，用于食品、化妆品和局部治疗制剂。它还对几种类型的癌细胞发挥细胞毒活性，包括前列腺癌细胞。然而，人们对薄荷醇抗前列腺癌作用的机制知之甚少。在这项研究中，我们利用DNA微阵列技术研究了薄荷醇对PC-3前列腺癌细胞基因表达谱的影响。基因集浓缩分析表明，薄荷醇主要影响细胞周期相关基因的表达。随后的实验分析证实，薄荷醇诱导G2/M期停滞。特别是，薄荷醇显著下调G2/M期进程的关键调节因子Polo-like kinase1(PLK1)的表达，并抑制其下游信号转导。我们的计算分析和实验验证为未来旨在阐明薄荷醇在前列腺癌细胞周期控制中的作用的研究提供了基础。(C)2012 Elsevier Inc.保留所有权利。
Menthol, a naturally occurring monoterpene, is used in foods, cosmetic products, and topical therapeutic preparations. It also exerts cytotoxic activity against several cancer cell types, including prostate cancer cells. However, little is known about the mechanism of menthol action against prostate cancer cells. In this study, we investigated the effect of menthol on the gene expression profile of PC-3 prostate cancer cells using DNA microarray analyses. Gene set enrichment analysis revealed that menthol primarily affects the expression of cell cycle-related genes. Subsequent experimental analyses validated that menthol induces G2/M arrest. Particularly, menthol markedly down-regulated polo-like kinase 1 (PLK1), a key regulator of G2/M phase progression and inhibited its downstream signaling. Our computational analyses and experimental validation provide a basis for future investigations that are aimed at elucidating the action of menthol on cell cycle control in prostate cancer cells. (c) 2012 Elsevier Inc. All rights reserved.