A phosphofructokinase B-type carbohydrate kinase family protein, PFKB1, is essential for chloroplast development at early seedling stage in rice.
A phosphofructokinase B-type carbohydrate kinase family protein, PFKB1, is essential for chloroplast development at early seedling stage in rice.
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DOI：
10.1016/j.plantsci.2019.110295
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发表时间：
2020-01
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通讯作者：
Xiaobo Zhu;Mu Ze;J. Yin;M. Chern;Mingrui Wang;Xiang Zhang;Rui Deng;Yongzhen Li;Haicheng Liao;Long Wang;Bin Tu;Linhua Song;M. He;Shigui Li;Wenming Wang;Xuewei Chen;Jing Wang;Weitao Li
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作者：
Xiaobo Zhu;Mu Ze;J. Yin;M. Chern;Mingrui Wang;Xiang Zhang;Rui Deng;Yongzhen Li;Haicheng Liao;Long Wang;Bin Tu;Linhua Song;M. He;Shigui Li;Wenming Wang;Xuewei Chen;Jing Wang;Weitao Li
在磷酸果糖激酶B型碳水化合物激酶（PCK）家族蛋白中，只有少数蛋白质，如果糖激酶样1和2，具有调节叶绿体发育的功能。在这里，我们报告的参与PCK蛋白PFKB 1在叶绿体发育的鉴定一个新的水稻突变体，可逆转的早期黄化Kitaake 2[rey（k2）]。突变体（k2）在幼苗早期表现出黄叶表型，光合色素减少，叶绿体发育迟缓，但在后期逐渐恢复。rey（k2）的表型是由PFKB 1蛋白的破坏引起的。Pfkb1基因广泛表达，其蛋白主要定位于叶绿体，在某些细胞中，定位于细胞核。此外，PFKB 1蛋白在体外具有磷酸果糖激酶活性。因此，k2突变体影响叶绿体相关基因的RNA水平。特别是，核编码RNA聚合酶（NEP）依赖性基因即使在变绿色后也以持续的高水平inrey（k2）表达，表明PFKB 1对于抑制NEP依赖性基因的表达是必需的。综上所述，我们的研究表明，PFKB 1作为一种新的调节器的早期叶绿体发育必不可少的功能，至少部分通过调节叶绿体相关基因。
Among the phosphofructokinase B-type carbohydrate kinase (PCK) family proteins, only few proteins, like the FRUCTOKINASE-LIKE 1 and 2, have been functionally characterized in regulation of chloroplast development. Here, we report the involvement of a PCK protein PFKB1 in chloroplast development by identification of a new rice mutant,revertible early yellowing Kitaake 2[rey(k2)]. The mutantrey(k2)shows yellow leaf phenotype, reduced photosynthetic pigments, and retarded chloroplast development during early stages of seedlings, but gradually recovered at later stages. The phenotype ofrey(k2)is resulted from the disruption of the PFKB1 protein. ThePfkb1gene is ubiquitously expressed, and its protein is mainly targeted to the chloroplast and, in some cells, to the nucleus. In addition, the PFKB1 protein possesses phosphofructokinase activityin vitro. Therey(k2)mutant affects RNA levels of chloroplast-associated genes. In particular, the nuclear-encoded RNA polymerase (NEP)-dependent genes are expressed at a sustained high level inrey(k2)even after turning green, indicating that PFKB1 is essential for suppressing the expression of NEP-dependent genes. Taken together, our study suggests that PFKB1 functions as a novel regulator indispensable for early chloroplast development, at least partly by regulating chloroplast-associated genes.