Multilocus sequence analysis of the genus Citrobacter and description of Citrobacter pasteurii sp nov.
Multilocus sequence analysis of the genus Citrobacter and description of Citrobacter pasteurii sp nov.
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DOI：
10.1099/ijs.0.000122
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发表时间：
2015-05-01
影响因子：
2.8
通讯作者：
Brisse, Sylvain
中科院分区：
文献类型：
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作者：
Clermont, Dominique;Motreff, Laurence;Brisse, Sylvain
通过基因rpoB、fusA、pyrG和leuS的内部部分测序、16S rRNA基因测序、基因组序列的平均核苷酸同一性（ANI）和生化测试，对来自不同来源并被分类为肠杆菌科柠檬酸杆菌属成员的菌株进行表征。系统发育分析的基础上的四个管家基因表明，11种柠檬酸杆菌属的有效公布的名称是很好地划定。菌株CIP 55.13（T）和CIP 55.9形成与柠檬酸杆菌相关的不同分支。CIP55.9和CIP55.13（T）之间的ANI为99.19%，而CIP55.13（T）和CIP105016（T）之间的ANI为94.75%。最接近的物种。生化特征巩固了这两个分离株代表一个单独的物种的事实，因此建议将其命名为Citrobacter pasteurii sp. nov.。模式菌株为CIP 55.13（T）（=DSM 28879（T）=Na 1a（T））。
Strains originating from various sources and classified as members of the genus Citrobacter within the family Enterobacteriaceae were characterized by sequencing internal portions of genes rpoB, fusA, pyrG and leuS, 16S rRNA gene sequencing, average nucleotide identity (ANI) of genomic sequences and biochemical tests. Phylogenetic analysis based on the four housekeeping genes showed that the 11 species of the genus Citrobacter with validly published names are well demarcated. Strains CIP 55.13(T) and CIP 55.9 formed a distinct branch associated with Citrobacter youngae. The ANI between CIP 55.9 and CIP 55.13(T) was 99.19%, whereas it was 94.75 % between CIP 55.13(T) and strain CIP 105016(T) of the species C. youngae, the most closely related species. Biochemical characteristics consolidated the fact that the two isolates represent a separate species, for which the name Citrobacter pasteurii sp. nov. is proposed. The type strain is CIP 55.13(T) (=DSM 28879(T)=Na 1a(T)).