Development and Application of a Polydimethylsiloxane-Based Passive Air Sampler to Assess Personal Exposure to SARS-CoV-2
Development and Application of a Polydimethylsiloxane-Based Passive Air Sampler to Assess Personal Exposure to SARS-CoV-2
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DOI：
10.1021/acs.estlett.1c00877
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发表时间：
2022-01-11
影响因子：
10.9
通讯作者：
Pollitt, Krystal J. Godri
中科院分区：
文献类型：
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作者：
Angel, Darryl M.;Gao, Dong;Pollitt, Krystal J. Godri
呼出的呼吸道飞沫和气溶胶可携带传染性病毒，是COVID-19的重要传播方式。最近的研究已经成功地检测空气中的SARS-CoV-2 RNA在室内设置使用主动采样方法。然而，这些采样器的成本、尺寸和维护限制了它们在高风险传播地区的长期监测能力。作为替代方案，无源采样器可以是小的、重量轻的和便宜的，并且不需要电力或维护以用于连续操作。将无源采样器集成到可穿戴设计中，可用于更好地了解个人对呼吸道病毒的暴露。本研究评估了使用基于聚二甲基硅氧烷（PDMS）的被动采样器评估个人暴露于气溶胶和液滴SARS-CoV-2的情况。在基于实验室的转鼓实验中测定PDMS上载病毒气溶胶的摄取速率，以估计被动采样器病毒载量的时间加权平均空气传播病毒浓度。被动采样器随后被嵌入可穿戴的夹子设计中，并分发给康涅狄格州的社区成员，以监测个人SARS-CoV-2暴露。在62名参与者中的5名（8%）佩戴的夹子上检测到病毒，个人暴露范围为4至112个SARS-CoV-2 RNA拷贝/m3，主要是在室内餐厅环境中。我们的研究结果表明，基于PDM的被动采样器可以作为一个有用的暴露评估工具，在现实世界的高风险环境中的空气传播的病毒暴露，并提供途径，为早期发现潜在的情况和指导特定地点的感染控制协议，先发制人的社区传播。
Exhaled respiratory droplets and aerosols can carry infectious viruses and are an important mode of transmission for COVID-19. Recent studies have been successful in detecting airborne SARS-CoV-2 RNA in indoor settings using active sampling methods. The cost, size, and maintenance of these samplers, however, limit their long-term monitoring ability in high-risk transmission areas. As an alternative, passive samplers can be small, lightweight, and inexpensive and do not require electrical power or maintenance for continual operation. Integration of passive samplers into wearable designs can be used to better understand personal exposure to the respiratory virus. This study evaluated the use of a polydimethylsiloxane (PDMS)-based passive sampler to assess personal exposure to aerosol and droplet SARS-CoV-2. The rate of uptake of virus-laden aerosol on PDMS was determined in lab-based rotating drum experiments to estimate time-weighted averaged airborne viral concentrations from passive sampler viral loading. The passive sampler was then embedded in a wearable clip design and distributed to community members across Connecticut to surveil personal SARS-CoV-2 exposure. The virus was detected on clips worn by five of the 62 participants (8%) with personal exposure ranging from 4 to 112 copies of SARS-CoV-2 RNA/m(3), predominantly in indoor restaurant settings. Our findings demonstrate that PDMS-based passive samplers may serve as a useful exposure assessment tool for airborne viral exposure in real-world high-risk settings and provide avenues for early detection of potential cases and guidance on site-specific infection control protocols that preempt community transmission.