Growth and Quantification of MERS-CoV Infection.
Growth and Quantification of MERS-CoV Infection.
复制标题
DOI：
10.1002/9780471729259.mc15e02s37
复制
发表时间：
2015-05-01
影响因子：
--
通讯作者：
Frieman MB
中科院分区：
文献类型：
--
作者：
Coleman CM;Frieman MB
中东呼吸综合征冠状病毒（MERS - CoV）是一种新兴的高致病性呼吸道病毒。虽然MERS - CoV在2012年才出现，但我们和其他人已经开发出了体外培养和量化传染性MERS - CoV和RNA复制产物的方法。MERS - CoV能够感染一系列细胞类型，但在Vero E6细胞中复制到高滴度。提出了MERS - CoV的传播和定量方法。©2015 by John Wiley & Sons, Inc。
Middle East respiratory syndrome coronavirus (MERS‐CoV) is an emerging highly pathogenic respiratory virus. Although MERS‐CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS‐CoV and RNA products of replication in vitro. MERS‐CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS‐CoV are presented. © 2015 by John Wiley & Sons, Inc.