Accelerated hypertrophic chondrocyte kinetics in GDF-7 deficient murine tibial growth plates.
Accelerated hypertrophic chondrocyte kinetics in GDF-7 deficient murine tibial growth plates.
复制标题
GDF-7 缺陷的小鼠胫骨生长板中加速肥大软骨细胞动力学。
DOI：
10.1002/jor.20574
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Hunziker,ErnstB
中科院分区：
文献类型：
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作者：
Mikic,Borjana;Ferreira,MariaP;Battaglia,ToddC;Hunziker,ErnstB
生长/分化因子（GDFs）是骨形态发生蛋白（BMPs）的一个亚群，以其在关节形成和软骨形成中的作用而闻名。最近的研究表明，缺乏其中一种信号分子GDF - 5的小鼠，由于肥厚期持续时间明显延长，胫骨近端软骨内骨生长速率降低。GDF‐7是一个相关的家族成员，它与GDF‐5具有高度的序列一致性。本研究的目的是确定GDF - 7缺乏是否也会改变小鼠的软骨内骨生长速率，如果是的话，是如何实现的。研究了5周龄雌性GDF‐7−/−小鼠和野生型对照幼崽胫骨近端生长板的体视学和细胞动力学参数。GDF‐7缺乏导致生长速率（+26%,p= 0.0084）和软骨连接处细胞损失率（+25%,p= 0.0217）显著增加。与野生型对照相比，GDF‐7缺陷小鼠的细胞也表现出明显更短的肥厚期持续时间（- 27%;p= 0.0326）。这些数据表明，在缺乏GDF‐7的情况下，软骨内骨生长的速度通过调节生长板软骨细胞的肥厚期持续时间而受到影响。这些发现进一步支持了越来越多的证据，表明GDFs与健康软骨的形成、成熟和维持有关。©2008骨科研究学会。Wiley期刊公司出版。[J]中华口腔医学杂志，2008
The Growth/Differentiation Factors (GDFs) are a subgroup of the Bone Morphogenetic Proteins (BMPs) well known for their role in joint formation and chondrogenesis. Mice deficient in one of these signaling molecules, GDF‐5, have recently been shown to exhibit a decreased rate of endochondral bone growth in the proximal tibia due to a significantly longer hypertrophic phase duration. GDF‐7 is a related family member, which exhibits a high degree of sequence identity with GDF‐5. The purpose of the present study was to determine whether GDF‐7 deficiency also alters the endochondral bone growth rate in mice and, if so, how this is achieved. Stereologic and cell kinetic parameters in proximal tibial growth plates from 5‐week‐old female GDF‐7 −/− mice and wild type control littermates were examined. GDF‐7 deficiency resulted in a statistically significant increase in growth rate (+26%;p= 0.0084) and rate of cell loss at the chondrosseous junction (+25%;p= 0.0217). Cells from GDF‐7 deficient mice also exhibited a significantly shorter hypertrophic phase duration compared to wild type controls (−27%;p= 0.0326). These data demonstrate that, in the absence of GDF‐7, the rate of endochondral bone growth is affected through the modulation of hypertrophic phase duration in growth plate chondrocytes. These findings further support a growing body of evidence implicating the GDFs in the formation, maturation, and maintenance of healthy cartilage. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:986–990, 2008