High fluence light emitting diode-generated red light modulates characteristics associated with skin fibrosis.
High fluence light emitting diode-generated red light modulates characteristics associated with skin fibrosis.
复制标题
发射二极管生成的红光调节与皮肤纤维化相关的特征。
DOI：
10.1002/jbio.201600059
复制
发表时间：
2016-12
影响因子：
2.8
通讯作者：
Jagdeo, Jared
中科院分区：
文献类型：
--
作者：
Mamalis, Andrew;Koo, Eugene;Garcha, Manveer;Murphy, William J.;Isseroff, R. Rivkah;Jagdeo, Jared
皮肤纤维化，通常称为皮肤疤痕，是一个重大的国际健康问题，治疗选择有限。皮肤纤维化的标志是成纤维细胞增殖、胶原蛋白产生和迁移速度增加。最近发表的临床观察表明可见红光可以改善皮肤纤维化。在这项研究中，我们假设高通量发光二极管产生的红光 (HF-LED-RL) 通过减少细胞增殖、胶原蛋白产生和人类皮肤成纤维细胞的迁移速度来调节皮肤纤维化的关键细胞特征。在此，我们证明 HF-LED-RL 可以增加活性氧 (ROS) 的生成长达 4 小时，抑制成纤维细胞增殖而不增加细胞凋亡，抑制胶原蛋白生成，并通过调节磷酸肌醇 3-激酶 (PI3K)/Akt 途径抑制迁移速度。我们证明 HF-LED-RL 能够抑制参与皮肤纤维化的统一细胞过程，包括成纤维细胞增殖、胶原蛋白产生和迁移速度。这些发现表明 HF-LED-RL 可能代表一种治疗皮肤纤维化的新方法。 LED 的优点包括低成本、便携性和易用性。进一步表征 HF-LED-RL 对成纤维细胞的光生物调节作用并研究 HF-LED-RL 在人类受试者中的抗纤维化作用可能会为这种皮肤纤维化治疗方法的实用性提供新的见解。
Skin fibrosis, often referred to as skin scarring, is a significant international health problem with limited treatment options. The hallmarks of skin fibrosis are increased fibroblast proliferation, collagen production, and migration speed. Recently published clinical observations indicate that visible red light may improve skin fibrosis. In this study we hypothesize that high-fluence light-emitting diode-generated red light (HF-LED-RL) modulates the key cellular features of skin fibrosis by decreasing cellular proliferation, collagen production, and migration speed of human skin fibroblasts. Herein, we demonstrate that HF-LED-RL increases reactive oxygen species (ROS) generation for up to 4 hours, inhibits fibroblast proliferation without increasing apoptosis, inhibits collagen production, and inhibits migration speed through modulation of the phosphoinositide 3-kinase (PI3K)/Akt pathway. We demonstrate that HF-LED-RL is capable of inhibiting the unifying cellular processes involved in skin fibrosis including fibroblast proliferation, collagen production, and migration speed. These findings suggest that HF-LED-RL may represent a new approach to treat skin fibrosis. LED advantages include low cost, portability, and ease of use. Further characterizing the photobiomodulatory effects of HF-LED-RL on fibroblasts and investigating the anti-fibrotic effects of HF-LED-RL in human subjects may provide new insight into the utility of this therapeutic approach for skin fibrosis.
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影响因子：
3.6
作者：
Lim AF;Weintraub J;Kaplan EN;Januszyk M;Cowley C;McLaughlin P;Beasley B;Gurtner GC;Longaker MT
通讯作者：
Longaker MT
影响因子：
3.3
作者：
Campbell, S. M.;Tyrrell, J.;Curnow, A.
通讯作者：
Curnow, A.
影响因子：
2.3
作者：
Sadick, Neil S
通讯作者：
Sadick, Neil S
影响因子：
3.7
作者：
Kanazawa S;Fujiwara T;Matsuzaki S;Shingaki K;Taniguchi M;Miyata S;Tohyama M;Sakai Y;Yano K;Hosokawa K;Kubo T
通讯作者：
Kubo T
影响因子：
6.5
作者：
Chen, J;Hoffman, BB;Isseroff, RR
通讯作者：
Isseroff, RR