CRISPR/Cas Precisely Regulated DNA-Templated Silver Nanocluster Fluorescence Sensor for Meat Adulteration Detection
CRISPR/Cas Precisely Regulated DNA-Templated Silver Nanocluster Fluorescence Sensor for Meat Adulteration Detection
复制标题
用于肉类掺假检测的 CRISPR/Cas 精确调控 DNA 模板银纳米簇荧光传感器
DOI：
10.1021/acs.jafc.2c04500
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发表时间：
2022
影响因子：
6.1
通讯作者：
Guoliang Li
中科院分区：
文献类型：
--
作者：
Jiahui Chen;Jianghua Liu;Di Wu;Ruiyuan Pan;Jian Chen;Yongning Wu;Mingquan Huang;Guoliang Li
肉类掺假会导致消费者欺诈、食物过敏和宗教问题。因此，迫切需要快速、灵敏的检测方法来监督肉品的真实性。本研究设计了一种基于CRISPR/Cas的DNA模板银纳米簇（DNA-AgNC）传感器，用于检测肉类掺假。CRISPR/Cas 12 a的特异性序列识别允许准确识别靶DNA。DNA-AgNCs是一类新型的无标记荧光探针，具有良好的光稳定性和荧光增强特性，可作为CRISPR/Cas 12 a系统的底物，通过精确调控Cas 12 a独特的靶DNA激活的反式切割活性，实现灵敏的扩增信号输出。 基于CRISPR/Cas 12 a的这种特异性识别、高效的信号转导以及DNA-AgNC的优异荧光特性，该方法在10 pM ~ 1 μM范围内具有良好的线性关系，检测限（LOD）低至1.9 pM，可实现肉类掺假的灵敏检测。
Meat adulteration can cause consumer fraud, food allergies, and religious issues. Rapid and sensitive detection methods are urgently demanded to supervise meat authenticity. Herein, a clustered regularly interspaced short palindromic repeats (CRISPR)/Cas precisely regulated DNA-templated silver nanocluster (DNA-AgNC) sensor was ingeniously designed to detect meat adulteration. Specific sequence recognition of CRISPR/Cas12a allowed accurate identification of target DNA. The emerging label-free fluorescent probes, DNA-AgNCs, a class of promising fluorophores in biochemical analysis with attractive photostability and remarkably enhanced fluorescence properties, were first introduced as the substrates of CRISPR/Cas12a system, allowing a sensitive output of amplified signals through the precise regulation of the unique target DNA-activatedtrans-cleavage activity of Cas12a. Based on this specific recognition, efficient signal transduction of CRISPR/Cas12a, and the outstanding fluorescence properties of DNA-AgNCs, the proposed strategy achieved a satisfactory linear range from 10 pM to 1 μM with a limit of detection (LOD) as low as 1.9 pM, which can achieve sensitive detection of meat adulteration.