Different genotoxic profiles between depleted and enriched uranium
Different genotoxic profiles between depleted and enriched uranium
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DOI：
10.1016/j.toxlet.2009.11.009
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发表时间：
2010-02-15
影响因子：
3.5
通讯作者：
Petitot, F.
中科院分区：
文献类型：
--
作者：
Darolles, C.;Broggio, D.;Petitot, F.
铀是一种发射α粒子的重金属，其遗传毒性来自其化学和放射性特性，这些特性随其同位素组成而变化（U-235（EU）中12%的浓缩铀比U-235（DU）中03%的贫化铀的比活度高20倍）铀的同位素组成对其遗传毒性的影响本研究用微核着丝粒分裂阻断试验评价了铀的遗传毒性。50 μ M和500 μ M）细胞接受低浓度（损失范围从0.3 μ戈伊到760 5 μ戈伊。具有一个微核的双核细胞的频率随着DU和EU两者浓度的增加而增加，以与EU比DU诱导更多的着丝粒阴性微核和核质桥相同的方式。这项研究表明，铀的遗传毒性特征取决于其同位素组成，DU和EU分别是低和高的致染色体断裂剂，然而，DU的非整倍体效应仍然很高。因此，有必要研究DU的非整倍体效应在与铀内暴露相关的致癌风险评估中的潜在作用（C）2009 Elsevier爱尔兰有限公司版权所有
Uranium is an alpha-particle-emitting heavy metal Its genotoxicity results from both its chemical and its radiological properties that vary with its isotopic composition (12% enriched Uranium in U-235 (EU) has a specific activity 20 times higher than 0 3% depleted uranium in U-235 (DU)) The influence of the isotopic composition of uranium on its genotoxic profile (clastogenic/aneugenic) has never been described The present study evaluated genotoxic profile Of Uranium with the cytokinesis-block micronuclues centromere assay C3H10T1/2 mouse embryo fibroblasts were contaminated with either DU or EU at different concentrations (5 mu M. 50 mu M and 500 mu M) Cells received low (loses ranging from 0.3 mu Gy to 760 5 mu Gy The frequency of binucleated cells with one micronucleus increased with increasing concentrations of both DU and EU in the same way EU induced more centromere-negative micronuclei and nucleoplasmic bridges than DU A correlation between these two clastogenic markers and ionizing radiation doses was observed Finally, this study showed that the genotoxic profile of uranium depends on its isotopic composition DU and EU are low and high clastogens, respectively However, DU aneugenic effects remain high Thus, there is a need to study the potential role of aneugenic effects of DU in carcinogenic risk assessment linked to uranium internal exposure (C) 2009 Elsevier Ireland Ltd All rights reserved