PP13 mRNA Expression in Trophoblasts From Preeclamptic Placentas
PP13 mRNA Expression in Trophoblasts From Preeclamptic Placentas
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DOI：
10.1177/1933719108328615
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发表时间：
2009-04-01
影响因子：
2.9
通讯作者：
Farina, Antonio
中科院分区：
文献类型：
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作者：
Sekizawa, Akihiko;Purwosunu, Yuditiya;Farina, Antonio
为了评估胎盘蛋白13 （PP13）在子痫前期发病中的作用，我们研究了PP13信使RNA在妊娠早期和晚期胎盘滋养细胞中的表达。首先，通过激光显微解剖，从有和没有先兆子痫的妊娠晚期胎盘冷冻切片中分离出合胞滋养细胞和胞外滋养细胞。合胞滋养细胞中PP13水平显著高于胞外滋养细胞。子痫前期患者的合体滋养细胞表达水平明显低于正常人。接下来，在妊娠11周时绒毛膜绒毛取样残余样本的滋养细胞中评估PP13的表达，随后发生先兆子痫。子痫前期组的水平明显低于正常病例。这些发现提示滋养细胞中pp13信使RNA表达的改变可能与子痫前期的发病机制有关。
To assess the role of placental protein 13 (PP13) in the pathogenesis qfpreeclampsia, the messenger RNA expression of PP13 was investigated in the trophoblasts from first and third trimester placenta. First, syncytiotrophoblasts and extravillous trophoblasts were separated from frozen section of third trimester placenta obtained from pregnant women with and without preeclampsia by laser microdissection. The PP13 levels of syncytiotrophoblasts were significantly higher than those of extravillous trophoblasts. The expression levels in syncytiotrophoblasts from the preeclampsia were significantly lower than those from normal. Next, the PP13 expression was assessed in trophoblasts from residual samples of chorionic villus sampling at 11 weeks of gestation, who subsequently developed preeclampsia. The levels in the preeclampsia group were significantly lower than those of normal cases. These findings indicate that art alteration in the PP13-messenger RNA expression in the trophoblasts may be associated with the pathogenesis of preeclampsia.