High potassium-induced glutamate release in the cochlea: in vivo microdialysis study combined with on-line enzyme fluorometric detection of glutamate
High potassium-induced glutamate release in the cochlea: in vivo microdialysis study combined with on-line enzyme fluorometric detection of glutamate
复制标题
高钾诱导的耳蜗谷氨酸释放：体内微透析研究与谷氨酸在线酶荧光检测相结合
DOI：
10.1016/s0006-8993(98)00330-8
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发表时间：
1998
期刊：
影响因子：
2.9
通讯作者：
S. Komune
中科院分区：
文献类型：
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作者：
K. Matsuda;Y. Ueda;A. Haruta;T. Tono;S. Komune
本文研究了高钾诱发豚鼠耳蜗去极化过程中外淋巴谷氨酸释放的时程变化及其钙依赖性。谷氨酸浓度连续分析酶联荧光测定结合微透析。两个峰值的谷氨酸增加被发现在响应灌注10分钟。在没有钙离子，第一个峰值被削弱，而第二个峰值的抑制是最小的。
The time course of changes in perilymphatic glutamate release and their Ca2+-dependency were studied in the guinea pig cochlea during high K+-evoked depolarization. The glutamate concentration was analyzed continuously by an enzyme-linked fluorometric assay combined with microdialysis. Two peaks of glutamate increase were found in response to perfusion for 10 min. In the absence of Ca2+, the first peak was diminished, whereas the inhibition of the second peak was minimal.
中原大一郎：大脑研究。
DOI：
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发表时间：
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期刊：
影响因子：
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作者：
通讯作者：
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