Free Fatty Acids and Methyl Jasmonate Trigger Defense Reactions in Laminaria digitata
Free Fatty Acids and Methyl Jasmonate Trigger Defense Reactions in Laminaria digitata
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DOI：
10.1093/pcp/pcp023
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发表时间：
2009-04-01
影响因子：
4.9
通讯作者：
Potin, Philippe
中科院分区：
文献类型：
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作者：
Kuepper, Frithjof C.;Gaquerel, Emmanuel;Potin, Philippe
花生四烯酸，亚麻酸和茉莉酸甲酯（MeJA）被发现是海带氧化爆发的强烈触发剂。这些研究结果构成了第一份报告的氧化爆发在藻类系统诱导的游离脂肪酸。活性氧物质的来源可以至少部分地被二苯基碘鎓（DPI）抑制。用花生四烯酸处理增加了许多游离脂肪酸[包括肉豆蔻酸（C14：0）、亚油酸（C18：2）、亚麻酸（C18：3）和二十碳五烯酸（C20：5）]和羟基化衍生物[如15-羟基二十碳四烯酸（15-HETE）、13-羟基十八碳三烯酸（13-HOTE）和15-羟基二十碳五烯酸（15-HEPE）]的水平。类似于先前报道的藻酸盐寡糖引发的氧化爆发在建立L. C20：4和MeJA诱导的氧化爆发似乎参与了在L.毛地黄总之，这项研究支持的概念，脂质氧化信号在防御诱导海洋褐藻中起着关键作用。
Arachidonic acid, linolenic acid and methyl jasmonate (MeJA) were found to be strong triggers of an oxidative burst in the kelp Laminaria digitata. These findings constitute the first report of an oxidative burst in an algal system induced by free fatty acids. The source of reactive oxygen species can be at least partially inhibited by diphenylene iodonium (DPI). Treatment with arachidonic acid increases the levels of a number of free fatty acids [including myristic (C14:0), linoleic (C18:2), linolenic (C18:3) and eicosapentaeneoic (C20:5) acids] and hydroxylated derivatives [such as 15-hydroxyeicosate-traenoic acid (15-HETE), 13-hydroxyoctadecatrienoic acid (13-HOTE) and 15-hydroxyeicosapentaenoic acid (15-HEPE)]. Similar to a previous report of the function of an alginate oligosaccharide-triggered oxidative burst in the establishment of resistance in L. digitata against infection by its brown algal endophyte Laminariocolax tomentosoides, C20:4- and MeJA-induced oxidative bursts seem to be involved in establishing the same protection in L. digitata. Altogether, this study supports the notion that lipid oxidation signaling plays a key role in defense induction in marine brown algae.