Multitoxin biosensor-mass spectrometry analysis: a new approach for rapid, real-time, sensitive analysis of staphylococcal toxins in food
Multitoxin biosensor-mass spectrometry analysis: a new approach for rapid, real-time, sensitive analysis of staphylococcal toxins in food
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DOI：
10.1016/s0168-1605(00)00328-7
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发表时间：
2000-09-15
影响因子：
5.4
通讯作者：
Nelson, RW
中科院分区：
文献类型：
--
作者：
Nedelkov, D;Rasooly, A;Nelson, RW
应用生物分子相互作用质谱法（BIA-MS）检测食品样品中的细菌毒素。这种两步方法利用表面等离子体共振（SPR）来检测毒素与固定在传感器芯片表面的抗体的结合。SPR检测之后，用基质辅助激光解吸/电离飞行时间质谱法鉴定结合毒素。在牛奶和蘑菇样品中检测到1 ng/ml的葡萄球菌肠毒素B （SEB）。此外，检测到食物成分与固定化抗体和传感器芯片表面的非特异性结合。为了评估BIA-MS在分析含有多种有毒成分的材料中的适用性，对同时含有SEE和中毒性休克综合征毒素- 1的样品进行了分析。利用多亲和传感器芯片表面成功地同时检测了两种毒素。(C) 2000 Elsevier Science B.V.版权所有
Biomolecular interaction analysis mass spectrometry (BIA-MS) was applied to detection of bacterial toxins in food samples. This two-step approach utilizes surface plasmon resonance (SPR) to detect the binding of the toxin(s) to antibodies immobilized on a surface of a sensor chip. SPR detection is then followed by identification of the bound toxin(s) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Staphylococcal enterotoxin B (SEB) was readily detected in milk and mushroom samples at levels of 1 ng/ml. In addition, non-specific binding of food components to the immobilized antibody and to the sensor chip surface was detected. To evaluate the applicability of BIA-MS in the analysis of materials containing multiple toxic components, sample containing both SEE and toxic-shock syndrome toxin-l was analyzed. Both toxins were successfully and simultaneously detected through the utilization of multiaffinity sensor chip surfaces. (C) 2000 Elsevier Science B.V. All rights reserved.