Neural stem cell-derived factors inhibit the growth and invasion of U87 stem-like cells in vitro
Neural stem cell-derived factors inhibit the growth and invasion of U87 stem-like cells in vitro
复制标题
神经干细胞衍生因子体外抑制U87干细胞样细胞的生长和侵袭
DOI：
10.1002/jcb.27826
复制
发表时间：
2019-04-01
影响因子：
4
通讯作者：
Liu, Yong
中科院分区：
文献类型：
--
作者：
Li, Xingxing;Tan, Ruolan;Liu, Yong
胶质瘤是脑内最常见和最具侵袭性的肿瘤之一。神经干/祖细胞（NSC/NPCs）作为治疗胶质瘤的载体的潜在用途受到了广泛关注。然而，NSC/NPC或它们产生的因子是否能做出贡献仍有待观察。本研究重点探讨了神经干细胞/NPC产生的因子对胶质瘤干样细胞体外生物学行为的抑制作用。选择人神经胶质瘤细胞系U87，并研究U87干细胞样细胞。在NSC条件培养基（NSC-CM）中培养后，U87干细胞样细胞的活力和增殖显著降低。U87干细胞样细胞的侵袭和U87细胞的迁移也显著降低。然而，在U87干细胞样细胞的星形胶质细胞分化方面没有观察到显著变化。这表明NSC/NPCs产生了一些因子，对U87干细胞样细胞的生长和侵袭有抑制作用，但对终末分化无抑制作用。NSCs/NPCs作为胶质瘤治疗的高潜力候选细胞值得关注。
Glioma is one of the most common and aggressive tumors in the brain. Significant attention has been paid to the potential use of neural stem/progenitor cells (NSCs/NPCs) as delivery vehicles to cure gliomas. However, whether the NSCs/NPCs or the factors they produced could make a contribution still remains to be seen. In this study, we focused on the inhibitory effects of the factors produced by NSCs/NPCs on the biological behavior of the glioma stem‐like cell in vitro. The human glioma cell line U87 was selected and the U87 stem‐like cells were addressed. After being cultured in the NSC condition medium (NSC‐CM), the viability and proliferation of U87 stem‐like cells were significantly reduced. The invasion of U87 stem‐like cells and the migration of U87 cells were also significantly decreased. However, no significant change was observed in regard to the astrocytic differentiation of U87 stem‐like cells. These indicated that NSCs/NPCs produced some factors and had an inhibitory effect on the growth and invasion but not the terminal differentiation of U87 stem‐like cells. It is worth paying attention to NSCs/NPCs as a high‐potential candidate for glioma treatment.