Expression of nestin after traumatic brain injury in rat brain
Expression of nestin after traumatic brain injury in rat brain
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DOI：
10.1016/s0006-8993(99)01757-6
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发表时间：
1999-09-04
期刊：
影响因子：
2.9
通讯作者：
Chopp, M
中科院分区：
文献类型：
--
作者：
Kaya, SS;Mahmood, A;Chopp, M
我们测试了这样的假设：创伤性脑损伤会上调巢蛋白（一种胚胎细胞中间丝蛋白）的表达。对受到控制性皮质冲击损伤的大鼠 (n = 24)、假手术大鼠 (n = 3) 和正常大鼠 (n = 3) 的大脑进行双标记免疫组织化学研究，以鉴定巢蛋白的细胞表达。我们的结果表明，在正常未受伤的动物中，巢蛋白轻微表达，并且仅定位于少数内皮细胞和室下细胞中。相反，损伤后 1-4 周，巢蛋白在星形胶质细胞和小胶质细胞中强烈表达。创伤性脑损伤后星形胶质细胞和小胶质细胞中巢蛋白的表达支持了以下假设：受伤的脑组织表达发育蛋白，并且这些蛋白可能促进损伤后的恢复。 (C) 1999 Elsevier Science B.V. 保留所有权利。
We tested the hypothesis that traumatic brain injury upregulates expression of nestin, an embryonic cell intermediate filament protein. Brain from rats (n = 24) subjected to controlled cortical impact injury and sham operated (n = 3) and normal(n = 3) rats were processed for dual label immunohistochemical study to identify cellular expression of nestin. Our results show that in normal noninjured animals, nestin is expressed slightly and localized only in a few endothelial and subventricular cells. In contrast, at 1-4 weeks postinjury nestin is strongly expressed in astrocytes and microglia. The expression of nestin in astrocytes and microglia after traumatic brain injury support the hypothesis that injured cerebral tissue expresses developmental proteins, and that these proteins may promote recovery after injury. (C) 1999 Elsevier Science B.V. All rights reserved.