Gm1-MMP is involved in growth and development of leaf and seed, and enhances tolerance to high temperature and humidity stress in transgenic Arabidopsis
Gm1-MMP is involved in growth and development of leaf and seed, and enhances tolerance to high temperature and humidity stress in transgenic Arabidopsis
复制标题
Gm1-MMP参与叶片和种子的生长发育，增强转基因拟南芥对高温高湿胁迫的耐受性
DOI：
10.1016/j.plantsci.2017.03.005
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发表时间：
2017-06-01
期刊：
影响因子：
5.2
通讯作者：
Ma, Hao
中科院分区：
文献类型：
--
作者：
Liu, Sushuang;Liu, Yanmin;Ma, Hao
基质金属蛋白酶(MMPs)是一类锌和钙依赖的内肽酶家族。研究发现，GM1-MMPs在大豆叶片展开过程中的组织重塑中起重要作用。本研究对GM1-MMPs进行了分离和鉴定。其编码蛋白与其他植物中的MMPs具有较低的亲缘关系。亚细胞定位结果表明GM1-MMPs是一种质膜蛋白。GML-MMPs在成熟叶片、老叶、豆荚和成熟种子中表达水平较高，并参与了大豆种子的发育。此外，它还参与了大豆R7叶片和种子对高温高湿胁迫的响应。对GML-MMP399~299片段启动子的分析表明，399~299片段是GML-MMP399~299启动子响应HTH胁迫所必需的。GM1-MMPs在拟南芥中的过表达影响了叶片的生长发育，提高了叶片和种子对HTH胁迫的耐受性，提高了种子的活力。在HTH胁迫下，转基因拟南芥种子的过氧化氢(H_2O_2)和ROS水平均低于野生型种子。酵母双杂交实验和BIFC实验证实GML-MMPs能与大豆金属硫蛋白-II(GmMT-II)相互作用。这些结果表明，GM1-MMPs在叶片和种子的生长发育以及对HTH胁迫的耐性中起着重要的作用。这将有助于我们理解GML-MMPs在植物生长发育和响应非生物胁迫中的作用。(C)2017爱思唯尔B.V.保留所有权利。
Matrix metalloproteinases (MMPs) are a family of zinc- and calcium-dependent endopeptidases. Gm1-MMP was found to play an important role in soybean tissue remodeling during leaf expansion. In this study, Gm1-MMP was isolated and characterized. Its encoding protein had a relatively low phylogenetic relationship with the MMPs in other plant species. Subcellular localization indicated that Gm1-MMP was a plasma membrane protein. Gml-MMP showed higher expression levels in mature leaves, old leaves, pods, and mature seeds, as well as was involved in the development of soybean seed. Additionally, it was involved in response to high temperature and humidity (HTH) stress in R7 leaves and seeds in soybean. The analysis of promoter of Gml-MMP suggested that the fragment from 399 to 299 was essential for its promoter activity in response to HTH stress. The overexpression of Gm1-MMP in Arabidopsis affected the growth and development of leaves, enhanced leaf and developing seed tolerance to HTH stress and improved seed vitality. The levels of hydrogen peroxide (H2O2) and ROS in transgenic Arabidopsis seeds were lower than those in wild type seeds under HTH stress. Gml-MMP could interact with soybean metallothionein-II (GmMT-II), which was confirmed by analysis of yeast two-hybrid assay and BiFC assays. All the results indicated that Gm1-MMP plays an important role in the growth and development of leaves and seeds as well as in tolerance to HTH stress. It will be helpful for us understanding the functions of Gml-MMP in plant growth and development, and in response to abiotic stresses. (C) 2017 Elsevier B.V. All rights reserved.