Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae.
Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae.
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信息素反应元件对于酿酒酵母 FUS1 基因的基础转录和信息素诱导转录是必要且充分的。
DOI：
10.1128/mcb.11.6.2952-2961.1991
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发表时间：
1991
影响因子：
5.3
通讯作者：
SpragueJr,GF
中科院分区：
文献类型：
--
作者：
Hagen,DC;McCaffrey,G;SpragueJr,GF
酿酒酵母的FUS1基因在a和α细胞中转录，而不是在a/α二倍体中转录，当单倍体细胞暴露于适当的交配信息素时，它的转录显著增加。此外，FUS1的转录完全依赖于STE4、STE5、STE7、STEH和STE12基因，这些基因被认为编码信息素反应途径的组成部分。我们现在已经确定，信息素反应元件(Pre)在FUS1上游区域以四个副本出现，起FUS1上游激活序列(UAS)的作用，负责FUS1调控的所有已知方面。特别是，包括PreS的55个碱基的缺失取消了所有转录，以及一个139个碱基的片段包括一个CyC1-lacZ报告基因的PreS相关FUS1样表达。此外，三到四个拷贝的合成PRE非常接近地模拟了139-bp片段赋予的活性，甚至一个拷贝的PRE也赋予了微量的单倍体特有的活性和信息素诱导的活性。在FUS1启动子的上下文中，在55bP缺失位置插入的合成Pre的四个副本恢复了FUS1的完整转录。Pre簇的上游和下游序列对于最大程度的预定向表达是重要的，但它们本身并不具有UAS活性。其他带有PreS的酵母基因，如STE2和BAR1，诱导能力较弱，并具有影响整体活性的额外UAS元件。在FUS1启动子中，PreS显然单独作用于赋予受到信息素高度刺激的活性。
TheFUS1gene ofSaccharomyces cerevisiaeis transcribed in a and α cells, not in a/α diploids, and its transcription increases dramatically when haploid cells are exposed to the appropriate mating pheromone. In addition,FUS1transcription is absolutely dependent onSTE4, STE5, STE7, STEH,andSTE 12,genes thought to encode components of the pheromone response pathway. We now have determined that the pheromone response element (PRE), which occurs in four copies within theFUS1upstream region, functions as theFUS1upstream activation sequence (UAS) and is responsible for all known aspects ofFUS1regulation. In particular, deletion of 55 bp that includes the PREs abolished all transcription, and a 139-bp fragment that includes the PREs conferredFUS1-like expression to aCYC1-lacZreporter gene. Moreover, three or four copies of a synthetic PRE closely mimicked the activity conferred by the 139-bp fragment, and even a single copy of PRE conferred a trace of activity that was haploid specific and pheromone inducible. In theFUS1promoter context, four copies of the synthetic PRE inserted at the site of the 55-bp deletion restored fullFUS1transcription. Sequences upstream and downstream from the PRE cluster were important for maximal PRE-directed expression but, by themselves, did not have UAS activity. Other yeast genes with PREs, e.g.,STE2andBAR1,are more modestly inducible and have additional UAS elements contributing to the overall activity. In theFUS1promoter, the PREs apparently act alone to confer activity that is highly stimulated by pheromone.