Efficient differentiation of human embryonic stem cells into a homogeneous population of osteoprogenitor-like cells
Efficient differentiation of human embryonic stem cells into a homogeneous population of osteoprogenitor-like cells
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DOI：
10.1016/s1472-6483(10)60490-7
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发表时间：
2008-05-01
影响因子：
4
通讯作者：
Sermon, Karen
中科院分区：
文献类型：
--
作者：
Mateizel, Ileana;De Becker, Ann;Sermon, Karen
人胚胎干细胞（hESC）在研究和治疗应用中的使用需要相对大的分化细胞的同质群体。本文报道了三种人胚胎干细胞系分化为高度同质的骨祖细胞样细胞（hESC-OPL）。这些细胞可以在特定的培养系统中扩增超过18代，并显示成纤维细胞样形态和正常稳定的核型。这些细胞对与间充质干细胞相同的抗原标记物呈强阳性，但对造血干细胞的标记物呈阴性。hESC-OPL细胞能够分化为成骨细胞，但不能分化为成软骨细胞或成脂细胞，并且成骨分化早期阶段的标志物呈阳性。当在成骨补充剂的存在下培养时，细胞表示在诱导条件下实现成熟成骨细胞表型的能力。分化方案基于单层方法，并且除了胎牛血清或动物或人来源的共培养系统之外不需要任何外源因子。这种方法可能适合于大规模生产同质的骨祖细胞样细胞，从而克服了hESC分化的主要问题之一，对于进一步的细胞治疗研究具有重要意义。
The use of human embryonic stem cells (hESC) in both research and therapeutic applications requires relatively large homogeneous populations of differentiated cells. The differentiation of three hESC lines into highly homogeneous populations of osteoprogenitor-like (hESC-OPL) cells is reported here. These cells could be expanded in a defined culture system for more than 18 passages, and showed a fibroblast-like morphology and a normal stable karyotype. The cells were strongly positive for the same antigenic markers as mesenchymal stem cells but negative for markers of haematopoetic stem cells. The hESC-OPL cells were able to differentiate into the osteogenic, but not into the chondrogenic or adipogenic, lineage and were positive for markers of early stages of osteogenic differentiation. When cultured in the presence of osteogenic supplements, the cells indicated the capacity to achieve, under inductive conditions, a mature osteoblast phenotype. The differentiation protocol is based on a monolayer approach, and does not require any exogenous factors other than fetal calf serum, or coculture systems of animal or human origin. This method is likely to be amenable to large-scale production of homogeneous osteoprogenitor-like cells and thus overcomes one of the major problems of differentiation of hESC, with important relevance for further cell therapy studies.