Molecular Detection of Toxoplasma Gondii in Haemaphysalis Ticks in Korea
Molecular Detection of Toxoplasma Gondii in Haemaphysalis Ticks in Korea
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DOI：
10.3347/kjp.2020.58.3.327
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发表时间：
2020-06-01
影响因子：
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通讯作者：
Yong, Tai-Soon
中科院分区：
文献类型：
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作者：
Kim, Ju Yeong;Kwak, You Shine;Yong, Tai-Soon
刚地弓形虫是一种细胞内的原生动物，可引起胎儿甚至免疫功能低下的成年人的神经系统疾病或死亡。蜱被认为是许多微生物的载体，包括病毒、细菌和原生动物。最近的研究发现T.在许多国家的各种蜱类中发现弓形虫。本研究采用PCR方法检测了T.从韩国元州、群山、米良和阳三4个地区的植被中采集的血蜱属蜱的弓形虫B1基因。对314只蜱（长头血蜱268只，黄血蜱46只）的DNA和T.其中13例检出弓形虫。T.不同地区蜱虫感染弓形虫率差异显著（P= 0.021）。T.在采集的蜱中检测到弓形虫的百分比如下：群山3.7%（189只中的7只）、元州10%（50只中的5只）、阳三16.7%（6只中的1只）和米良0%（69只中的0只）。T.蜱体内弓形虫感染与蜱种或发育阶段无关。这是T.韩国蜱虫中的弓形虫检测。我们的研究结果提供了重要的信息，了解弓形虫病的传播。
Toxoplasma gondii are intracellular protozoa that can cause neurological disease or death in fetuses and even in immunocompromised human adults. Ticks are recognized as vectors of many microorganisms including viruses, bacteria, and protozoa. Recent studies detected T. gondii in various tick species in many countries. In this study, we performed PCR detection of the T. gondii B1 gene from Haemaphysalis ticks collected from vegetation in 4 localities, Wonju, Gunsan, Miryang, and Yangsan, in Korea. We analyzed DNA from 314 ticks (268 Haemaphysalis Iongicomis and 46 Haemaphysalis flava) and the B1 gene of T. gondii was detected in 13 of these. The detection of T. gondii in ticks differed significantly by region (P= 0.021). T. gondii was detected in the following percentages of collected ticks: 3.7% (7 of 189) in Gunsan, 10% (5 of 50) in Wonju, 16.7% (1 of 6) in Yangsan, and 0% (0 of 69) in Miryang. The detection of T. gondii in ticks was not associated with tick species or development stage. This is the first report of T. gondii detection in ticks in Korea. Our results provide important information necessary to understand toxoplasmosis transmission.