Bacterial-induced calcium oscillations are common to nitrogen-fixing associations of nodulating legumes and nonlegumes.
Bacterial-induced calcium oscillations are common to nitrogen-fixing associations of nodulating legumes and nonlegumes.
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DOI：
10.1111/nph.13464
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发表时间：
2015-08
期刊：
影响因子：
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通讯作者：
Oldroyd GE
中科院分区：
文献类型：
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作者：
Granqvist E;Sun J;Op den Camp R;Pujic P;Hill L;Normand P;Morris RJ;Downie JA;Geurts R;Oldroyd GE
形成根瘤共生的植物是在一个单系的“固氮”分支内，相关的信号传导过程与丛枝菌根共生共享。共生信号的核心是钙离子（Ca2+）的核相关振荡，发生在几种豆科植物的根毛中，以响应根瘤菌结瘤因子信号。在这项研究中，我们扩展了分析激活Ca2+振荡的物种，包括固氮分支中的非豆科物种。我们发现，Ca2+振荡是豆科植物与根瘤菌结合的一个共同特征，而紫荆，一种不结瘤的豆科植物，对来自费氏中华根瘤菌NGR 234的结瘤因子没有表现出Ca2+振荡。一种可以与根瘤菌结合的非豆科植物，Parasponia andersonii，对S. fredii NGR 234 Nod因子显示出Nod因子诱导的钙振荡，但其非繁殖的姐妹物种Trema tomentosa没有。此外，在固氮进化枝内是与Frankia细菌相关的放线菌物种，并且我们表明，赤杨诱导根毛中的Ca2+振荡以响应Frankia alni的分泌物，但不响应S. fredii NGR234 Nod因子。我们的结论是，响应共生细菌安装Ca2+振荡的能力是固氮进化枝内的微生物物种的共同特征。
Plants that form root‐nodule symbioses are within a monophyletic ‘nitrogen‐fixing’ clade and associated signalling processes are shared with the arbuscular mycorrhizal symbiosis. Central to symbiotic signalling are nuclear‐associated oscillations in calcium ions (Ca2+), occurring in the root hairs of several legume species in response to the rhizobial Nod factor signal. In this study we expanded the species analysed for activation of Ca2+ oscillations, including non‐leguminous species within the nitrogen‐fixing clade. We showed that Ca2+ oscillations are a common feature of legumes in their association with rhizobia, while Cercis, a non‐nodulating legume, does not show Ca2+ oscillations in response to Nod factors from Sinorhizobium fredii NGR234. Parasponia andersonii, a non‐legume that can associate with rhizobia, showed Nod factor‐induced calcium oscillations to S. fredii NGR234 Nod factors, but its non‐nodulating sister species, Trema tomentosa, did not. Also within the nitrogen‐fixing clade are actinorhizal species that associate with Frankia bacteria and we showed that Alnus glutinosa induces Ca2+ oscillations in root hairs in response to exudates from Frankia alni, but not to S. fredii NGR234 Nod factors. We conclude that the ability to mount Ca2+ oscillations in response to symbiotic bacteria is a common feature of nodulating species within the nitrogen‐fixing clade.