The crystal structure of myoglobin III. Sperm-whale myoglobin
The crystal structure of myoglobin III. Sperm-whale myoglobin
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肌红蛋白 III 的晶体结构。
DOI：
10.1098/rspa.1957.0002
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发表时间：
1957
期刊：
影响因子：
--
通讯作者：
R. Parrish
中科院分区：
文献类型：
--
作者：
J. Kendrew;R. Parrish
抹香鲸肌红蛋白在硫酸铵溶液中形成空间群为P21的单斜晶体（A型），在磷酸盐溶液中形成空间群为P21 21 21的斜方晶体（B型）。几乎相同的晶体形式是从鲸鱼的相关物种中获得的。二维帕特森投影已计算出一些主要区域的反射在每种类型的晶体。它们用于推导分子中多肽链相对于晶轴的链方向，并提出两种形式包装的合理方法。数据最适合于尺寸约为25 x 34 x 42 nm的分子，后者的尺寸平行于链方向。电子自旋共振（班尼特和英格拉姆，1956年）和光学二色性的测量已经被用来确定血红素基团的平面的取向和它们与链之间的角度。得出结论，这两种形式的角度为40 °至50°，与先前文献中F型晶体的值，即41°，非常一致。因此，在所有三种晶体类型A、B和F中，数据与具有相同尺寸组的分子一致，与共同的链方向以及链方向和血红素基团之间的共同关系相关。
Myoglobin of Physeter catodon (sperm whale) forms monoclinic crystals with space group P21 (type A) in ammonium sulphate, and orthorhombic crystals with space group P21 21 21 (type B) in phosphate. Almost identical crystal forms are obtained from related species of whale. Two-dimensional Patterson projections have been computed from some principal zones of reflexions in each type of crystal. They are used to derive the chain directions of the polypeptide chains in the molecules relative to the crystal axes, and to suggest plausible methods of packing in the two forms. The data are best fitted by a molecule having dimensions about 25 x 34 x 42 Å, the latter dimension being parallel to the chain direction. Measurements of electron-spin resonance (Bennett & Ingram 1956) and of optical dichroism have been used to determine the orientation of the planes of the haem group and the angle between them and the chains. It is concluded that the angle is 40 to 50° in the two forms, in good agreement with the value found for type F crystals in an earlier paper, namely, 41°. Thus in all three crystal types A, B and F the data are consistent with the molecule having the same set of dimensions, associated with a common chain direction and a common relation between chain direction and haem group.