Response properties of slow PIII in the Large (vls) mutant.
Response properties of slow PIII in the Large (vls) mutant.
复制标题
大 (vls) 突变体中慢速 PIII 的响应特性。
DOI：
10.1007/s10633-012-9347-9
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Sturgill-Short,GwenM
中科院分区：
文献类型：
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作者：
Peachey,NealS;Sturgill-Short,GwenM
PurposeMouse mutants for proteins expressed in the dystrophin–glycoprotein complex at the photoreceptor terminal have electroretinogram (ERG) b-waves with a delayed onset and time course. The b-wave is defined by the sum of PII generated by depolarizing bipolar cells and slow PIII generated by Müller glial cells. In this study, we evaluated the hypothesis that the abnormalities observed in one of these mutants,Largevls, are caused by abnormal response properties of slow PIII.MethodsTo isolate slow PIII, we crossed theLargevlsmutant to a mouse line (Gpr179nob5) that lacks the ERG b-wave but maintains normal photoreceptor function and in which retinal degeneration does not occur. ERGs were recorded to strobe flash stimuli after overnight dark adaptation.ResultsIn comparison with control responses, the a-wave and slow PIII had comparable waveforms but were reduced in amplitude inLargevlsmice. The magnitude of this reduction was comparable for these components, and across stimulus luminance. There was no stimulus condition where the amplitude of slow PIII was larger than control.ConclusionsThe data obtained are inconsistent with the idea that the b-wave abnormalities noted inLargevlsmutant mice are caused by abnormal response properties of slow PIII.
