The brinker gradient controls wing growth in Drosophila
The brinker gradient controls wing growth in Drosophila
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DOI：
10.1242/dev.01385
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发表时间：
2004-10-01
期刊：
影响因子：
4.6
通讯作者：
Morata, G
中科院分区：
文献类型：
--
作者：
Martín, FA;Pérez-Garijo, A;Morata, G
十肢麻痹（Dpp）形态梯度控制果蝇附肢的生长和图案化。最近有证据表明，Dpp梯度被转换成基因brinker（brk）的活性的反向梯度，该基因编码转录抑制因子，并受Dpp途径的负调控。我们已经研究了Brk梯度的改变如何影响翼盘的生长。我们发现brk活性和椎间盘生长之间存在负相关性：高水平的brk阻止或减少生长，而brk活性的丧失导致过度生长。这种效应是浓度依赖性的：不同量的Brk产生不同的生长速率。此外，我们的研究结果表明，虽然brk是能够诱导细胞凋亡的Brk水平有一个急剧的差异，其作为一个生长抑制剂的作用是不通过诱导细胞凋亡，但通过减少细胞增殖。Brk似乎下调了控制细胞增殖的基因（如bantam）的活性。
The Decapentaplegic (Dpp) morphogen gradient controls growth and patterning in the Drosophila appendages. There is recent evidence indicating that the Dpp gradient is converted into an inverse gradient of activity of the gene brinker (brk), which encodes a transcriptional repressor and is negatively regulated by the Dpp pathway. We have studied how alterations in the Brk gradient affect the growth of the wing disc. We find that there is a negative correlation between brk activity and growth of the disc: high levels of brk prevent or reduce growth, whereas loss of brk activity results in excessive growth. This effect is concentration dependent: different amounts of Brk produce distinct rates of growth. Furthermore, our results demonstrate that although brk is able to induce apoptosis where there is a sharp difference in Brk levels, its role as a growth repressor is not achieved by inducing apoptosis but by reducing cell proliferation. Brk appears to downregulate the activity of genes that control cell proliferation, such as bantam.