MEIOTIC MATURATION OF MOUSE OOCYTES TRIGGERS THE TRANSLATION AND POLYADENYLATION OF DORMANT TISSUE-TYPE PLASMINOGEN-ACTIVATOR MESSENGER-RNA
MEIOTIC MATURATION OF MOUSE OOCYTES TRIGGERS THE TRANSLATION AND POLYADENYLATION OF DORMANT TISSUE-TYPE PLASMINOGEN-ACTIVATOR MESSENGER-RNA
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DOI：
10.1101/gad.1.10.1201
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发表时间：
1987-12-01
影响因子：
10.5
通讯作者：
VASSALLI, JD
中科院分区：
文献类型：
--
作者：
HUARTE, J;BELIN, D;VASSALLI, JD
The serine protease tissue-type plasminogen activator (t-PA) is synthesized by murine oocytes undergoing meiotic maturation, but not by arrested primary oocytes. Dormant, stable t-PA mRNA accumulates during oocyte growth, so that fully grown, arrested primary oocytes contain in their cytoplasm approximately 10,000 copies of this molecule. Translation of t-PA mRNA is triggered upon resumption of meiosis and is accompanied by a progressive and concerted increase in its size. This structural change can be accounted for by increased polyadenylation at the 3' end of the molecule. Following its translation, t-PA mRNA is degraded; it is no longer detectable in fertilized eggs. The identification of a dormant mRNA in murine oocytes and the demonstration that its translational activation is accompanied by elongation of its poly(A) tail may provide insights into the control of gene expression during meiotic maturation and early mammalian development.
