Evidence for the involvement of endothelial nitric oxide synthase from smooth muscle cells in the erectile function of the human corpus cavernosum
Evidence for the involvement of endothelial nitric oxide synthase from smooth muscle cells in the erectile function of the human corpus cavernosum
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DOI：
10.1007/s002400050035
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发表时间：
1998-04-01
影响因子：
--
通讯作者：
Addicks, K
中科院分区：
文献类型：
--
作者：
Bloch, W;Klotz, T;Addicks, K
一氧化氮（NO）是海绵体平滑肌舒张的重要介质。本研究探讨的存在和位置的构型eNOS（内皮NO合酶）伴随着已经证实的神经源性NOS（nNOS）在人阴茎海绵体的男性和不勃起功能障碍。NOS酶的活性进行了检查标本中的11个有效的和9个长期阳痿患者通过光镜和电镜使用NADPH-黄递酶染色和免疫组化eNOS特异性，平滑肌肌动蛋白特异性和nNOS特异性标记。海绵体平滑肌显示eNOS的独特表达。与大静脉中eNOS和nitrinergic神经支配较弱的表达相反，海绵体内的小螺旋动脉表达大量的eNOS，并具有密集的nitrinergic神经支配。长期阳萎患者eNOS表达和一氧化氮能神经支配表现出广泛的异质性，而NOS表达和勃起功能之间没有观察到整体相关性。eNOS的表达表明eNOS与nNOS一起是NO的主要来源。eNOS的分化定位至少支持这种亚型在血管调节中的作用。
Nitric oxide (NO) is an important mediator in the relaxation of cavernosal smooth muscle. The present study examines the existence and location of the constitutive isoform eNOS (endothelial NO synthase) accompanying the already substantiated neurogenic NOS (nNOS) in the human corpus cavernosum of men with and without erectile dysfunction. Activities of NOS enzymes were examined in specimens of 11 potent and nine long-term impotent patients by means of light and electron microscopy using NADPH-diaphorase staining and immunohistochemical eNOS-specific, smooth muscle actin-specific and nNOS-specific markers. Cavernosal smooth muscle shows a distinct expression of eNOS. In contrast to the weaker expression of eNOS and nitrinergic innervation found in larger veins, the small intracavernosal helicine arteries express large quantities of eNOS and possess a dense nitrinergic innervation. Long-term impotent patients display a broad heterogeneity in eNOS expression and nitrinergic innervation while no overall correlation between NOS expression and erectile function was observed. The expression of eNOS indicates eNOS as a main source of NO alongside nNOS. The differentiated localization of eNOS supports at least a role of this isoform in vascular regulation.