Development of user-friendly markers for the pvr1 and Bs3 disease resistance genes in pepper
Development of user-friendly markers for the pvr1 and Bs3 disease resistance genes in pepper
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DOI：
10.1007/s11032-015-0260-2
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发表时间：
2015-01
影响因子：
3.1
通讯作者：
William L. Holdsworth;M. Mazourek
中科院分区：
文献类型：
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作者：
William L. Holdsworth;M. Mazourek
病毒和黄单胞菌属，细菌性斑点病病原菌对辣椒（Capsicumspp.）在美国生产。几十年来，辣椒种植者一直依赖宿主植物抗性作为抵御这些病原体的第一道防线，辣椒育种者已经部署，克隆和表征了越来越多的抗性基因。相对于需要病原体接种的方法，这些基因内或与这些基因连锁的分子标记促进了育种群体抗性等位基因的快速筛选。我们已经开发了用户友好的标记，在编码区的克隆pvr 1和Bs3抗性基因使用竞争等位基因特异性PCR（KASP）基因分型系统，以增加鲁棒性和吞吐量，这些位点进行筛选。KASP标记物价格便宜，处理速度快，并且容易评分。
Viruses andXanthomonasspp., the causal agent of bacterial spot, are serious threats to pepper (Capsicumspp.) production in the USA. For decades, pepper growers have relied on host plant resistance as a first line of defense against these pathogens, and pepper breeders have deployed, cloned, and characterized a growing number of resistance genes. Molecular markers within or linked to these genes have facilitated rapid screening of breeding populations for resistance alleles relative to methods requiring pathogen inoculation. We have developed user-friendly markers in coding regions for the clonedpvr1andBs3resistance genes using theKompetitive Allele-Specific PCR(KASP) genotyping system in order to increase the robustness and throughput by which these loci are screened. The KASP markers are inexpensive, fast to process, and easily scored.