SODIUM PLUS POTASSIUM TRANSPORT ADENOSINE TRIPHOSPHATASE ACTIVITY IN KIDNEY.
SODIUM PLUS POTASSIUM TRANSPORT ADENOSINE TRIPHOSPHATASE ACTIVITY IN KIDNEY.
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钠加钾在肾脏中转运三磷酸腺苷酶活性。
DOI：
10.1002/jcp.1030620110
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发表时间：
1963
影响因子：
--
通讯作者：
D. Beaver
中科院分区：
文献类型：
--
作者：
C. R. Kinsolving;R. Post;D. Beaver
在寻找钠转运的酶学基础时，Skou ('57) 在蟹神经颗粒中发现了一种独特的 Mg++ 激活的三磷酸腺苷酶 (ATPase) 活性，该活性需要钠离子和钾离子在一起。随后Post等人('60)在破损的红细胞膜中发现了类似的ATP酶活性，作为完整红细胞主动转运钠和钾的系统的一部分。鉴定是根据九个相应特征进行的。强心苷哇巴因的抑制作用就是其中之一。 Dunham 和 Glynn ('61)、Hoffman ('62) 和 Tosteson 等人 ('60) 通过不同标准进行了鉴定。 Bonting 等人('62) 发现八种不同组织中哇巴因敏感的 ATP 酶活性与哇巴因敏感的钠和钾转运之间存在定性相关性。该活性已在神经细胞膜（Bonting 和 Caravaggio，'62；Cummins 和 Hydkn，'62）、肝细胞膜（Emmelot 和 Bos，'62）、肠道（Taylor，'62）和心肌（Skou，'62；Auditore，'62；Yu 和 Lee，'62；Schwartz，'62）中发现。 Jarnefelt ('62)、Deul 和 McIlwain ('61)、Skou ('62)、Somogyi 和 Vincze ('62)、Aldridge ('62)、Yoshida 和 Fujisawa ('62) 以及 Schwartz、Bachelard 和 McIlwain ('62) 对大脑准备工作进行了详细研究。在肾脏中，Whittam 和 Wheeler ('62) 和 Landon ('62) 已表现出类似的活性，而 Skou 则表征了一种特别活性的制剂 ('62)。本文描述了活性相同但不同的制剂的特性。这些特性与人红细胞膜中 (Na++ K+) 转运 ATP 酶活性的特性非常接近，表明两种组织中主动钠和钾转运的基本机制是相同的。这项工作的初步报告已经出现（Kinsolving 和 Post，'60；Post，'61）。
In searching for an enzymatic basis for sodium transport Skou ('57) discovered in particles from crab nerve a unique Mg++-activated adenosine triphosphatase (ATPase) activity which required both sodium and potassium ions together. Subsequently Post et al.('60) identified a similar ATPase activity in broken erythrocyte membranes as part of the system for the active transport of sodium and potassium intact red cells. The identification was made on the basis of nine corresponding characteristics. Inhibition by the cardiac glycoside, ouabain, was one of these. Identification by different criteria was made by Dunham and Glynn ('61), Hoffman ('62), and Tosteson et al.('60). Bonting et al.('62) found a qualitative correlation between ouabain-sensitive ATPase activity and ouabain-sensitive sodium and potassium transport in eight different tissues. The activity has been found in nerve cell membranes (Bonting and Caravaggio,'62; Cummins and Hydkn,'62) liver cell membranes (Emmelot and Bos,'62), in intestine (Taylor,'62), and in heart muscle (Skou,'62; Auditore,'62; Yu and Lee,'62; Schwartz,'62). Detailed studies of brain preparations have been made by Jarnefelt ('62), Deul and McIlwain ('61), Skou ('62), Somogyi and Vincze ('62), Aldridge ('62), Yoshida and Fujisawa ('62), and Schwartz, Bachelard, and McIlwain ('62). In kidney Whittam and Wheeler ('62) and Landon ('62) have demonstrated a similar activity and Skou has characterized a particularly active preparation ('62). The present paper describes the properties of an equally active but different preparation. The properties correspond so closely with those of the (Na++ K+) transport ATPase activity in human erythrocyte membranes as to suggest that the basis mechanism of active sodium and potassium transport is the same in the two tissues. Preliminary reports of this work have appeared (Kinsolving and Post,'60; Post,'61).