FT-IR profiling reveals differential response of roots and leaves to salt stress in a halophyte Sesuvium portulacastrum (L.) L.
FT-IR profiling reveals differential response of roots and leaves to salt stress in a halophyte Sesuvium portulacastrum (L.) L.
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DOI：
10.1016/j.btre.2019.e00352
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发表时间：
2019-09-01
影响因子：
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通讯作者：
Suprasanna, P.
中科院分区：
文献类型：
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作者：
Nikalje, G. C.;Kumar, Jitendra;Suprasanna, P.
在盐生植物 Sesuvium portulacastrum (L.) L. 中，我们应用傅里叶变换红外 (FT-IR) 光谱来检测在 0、100 和 500 mM NaCl 长期处理下与盐诱导的生理变化相关的相应变化。 FT-IR 图谱显示，由于盐处理，蛋白质、脂质和碳水化合物的化学成分和官能团发生了变化，这些代谢物特有的根和叶的 FT-IR 图谱差异很明显。此外，应用主成分分析 (PCA) 来识别由于差异处理而导致 FT-IR 数据变化的主要来源。在 PCA 中，PC1 显示出 85.55%，PC2 显示出 18.18% 的数据变异性，并证实了根和叶对 Sesuvium 盐处理的不同反应。结果表明，傅立叶变换红外光谱法可用于研究植物中胁迫诱导的代谢变化与其耐盐性的关系。 (C) 2019 年由 Elsevier B.V. 出版
In a halophyte, Sesuvium portulacastrum (L.) L., we have applied Fourier Transform InfraRed (FT-IR) spectroscopy to detect the corresponding changes associated with salt-induced physiological changes under long- term treatment with 0, 100 and 500 mM NaCl. FT-IR profiles showed changes in chemical composition and functional groups of proteins, lipids and carbohydrates due to salt treatments, evident as differential FT-IR profiles in both roots and leaves specific to these metabolites. Further, the Principle Component Analysis (PCA) was applied to identify the main sources of variation in FT-IR data due to differential treatment. In PCA, the PC1 showed 85.55% and PC2 showed 18.18% variability in data and confirmed differential response of root and leaves to salt treatment in Sesuvium. The results suggest that FT-IR spectrometry can be used to study stress-induced metabolic changes in plants in relation to their salt tolerance. (C) 2019 Published by Elsevier B.V.