Phylogenetic analysis of the Agrocybe aegerita multispecies complex in Southwest China inferred from ITS and mtSSU rDNA sequences and mating tests
Phylogenetic analysis of the Agrocybe aegerita multispecies complex in Southwest China inferred from ITS and mtSSU rDNA sequences and mating tests
复制标题
ITS和mtSSU rDNA序列及交配试验推断中国西南地区茶树菇多物种复合体的系统发育分析
DOI：
10.1007/s13213-012-0437-4
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发表时间：
2012-12-01
影响因子：
3
通讯作者：
Zhao, Yong-Chang
中科院分区：
文献类型：
--
作者：
Chen, Wei-Min;Chai, Hong-Mei;Zhao, Yong-Chang
对云南省茶树菇多种复合体的多样性进行了研究。通过线粒体小亚基（mtSSU）rDNA序列分析，将来自10个地区的23个菌株分为3个类群。内转录间隔区（ITS）系统发育分析结合交配试验表明，供试菌株至少包括两个种。柳生茶树菇（Agrocybe salicacola）是柳生茶树菇（A. Aegerita complex，首次进行了系统的分析和分子鉴定。YAASM0594和YAASM0625与YAASM1024具有相同的序列，而B群中的XW01在ITS系统发育树中与C群的亲缘关系较近，表明B群与CS45群亲缘关系较近，甚至可能是同一种。我们认为，茶树菇复合体的多样性比以前报道的要高，通过分子遗传学和交配试验可能会发现更多的新物种。
The diversity of Agrocybe aegerita multispecies complex in Yunnan Province, southwest China was investigated. In total, 23 strains collected from ten districts were classified into three groups through mitochondrial small-subunit (mtSSU) rDNA sequence analysis. Phylogeny of internal transcribed spacer (ITS) combined with mating tests demonstrated that the tested strains included at least two species. Agrocybe salicacola, which is difficult to distinguish from other species in the A. aegerita complex, was first systematically analyzed and identified with molecular characterization. YAASM0594 and YAASM0625 share sequences with YAASM1024, and XW01 in group B, defined according to the mtSSU rDNA sequences, was related more closely to group C in the ITS phylogenetic tree, indicating that group B and CS45 are closely related, with both groups possibly even being the same species. We conclude that a high diversity occurs in Agrocybe aegerita complex than reported previously, and that more novel species would possibly be discovered through molecular phylogeny and mating tests.