Personal sampler for monitoring of viable viruses; modelling of outdoor sampling conditions
Personal sampler for monitoring of viable viruses; modelling of outdoor sampling conditions
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DOI：
10.1016/j.atmosenv.2006.06.006
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发表时间：
2006-11-01
影响因子：
5
通讯作者：
Agranovski, I. E.
中科院分区：
文献类型：
--
作者：
Borodulin, A. I.;Desyatkov, B. M.;Agranovski, I. E.
最近开发了一种新的个人生物气溶胶采样器，经验证，它对监测空气中的活细菌、真菌和病毒非常有效。该装置能够提供高回收率，甚至对于对物理和生物应力相当敏感的微生物。然而，考虑到目标微生物的灭活速率、采样参数和从采样器收集液体的微生物分析结果，需要一些数学程序来实际计算空气中活生物气溶胶的实际浓度。在本文中，我们发展了这样的程序沿着与模式的气溶胶传播的室外条件。将这些程序结合起来，就可以确定最佳取样地点，尽可能最好地覆盖待监测区域。考虑到俄罗斯新西伯利亚中心广场音乐会期间恐怖分子袭击的假设事件，以评估在广场内不同地点检测生物气溶胶的采样设备对该区域的可能覆盖范围。研究发现，对于选定的生物气溶胶生成参数和天气条件，新的个人采样器将能够可靠地检测人群占据的所有地点的病原体，即使距离源高达600米。(c)2006年由Elsevier Ltd.出版
A new personal bioaerosol sampler has recently been developed and verified to be very efficient for monitoring of viable airborne bacteria, fungi and viruses. The device is capable of providing high recovery rates even for microorganisms which are rather sensitive to physical and biological stresses. However, some mathematical procedure is required for realistic calculation of an actual concentration of viable bioaerosols in the air taking into account a rate of inactivation of targeted microorganisms, sampling parameters, and results of microbial analysis of collecting liquid from the sampler. In this paper, we develop such procedure along with the model of aerosol propagation for outdoor conditions. Combining these procedures allows one to determine the optimal sampling locations for the best possible coverage of the area to be monitored. A hypothetical episode concerned with terrorists' attack during music concert in the central square of Novosibirsk, Russia was considered to evaluate possible coverage of the area by sampling equipment to detect bioaerosols at various locations within the square. It was found that, for chosen bioaerosol generation parameters and weather conditions, the new personal sampler would be capable to reliably detect pathogens at all locations occupied by crowd, even at distances of up to 600m from the source. (c) 2006 Published by Elsevier Ltd.