The crystal structure of HIV CRF07 B′/C gp41 reveals a hyper-mutant site in the middle of HR2 heptad repeat
The crystal structure of HIV CRF07 B′/C gp41 reveals a hyper-mutant site in the middle of HR2 heptad repeat
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HIV CRF07 Bâ²/C gp41 的晶体结构揭示了 HR2 七肽重复序列中间的超突变位点
DOI：
10.1016/j.virol.2013.07.024
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发表时间：
2013-11-01
期刊：
影响因子：
3.7
通讯作者：
Liu, Xinqi
中科院分区：
文献类型：
--
作者：
Du, Jiansen;Xue, Hailing;Liu, Xinqi
CRF 07 B '/C是我国西北地区流行的HIV毒株。CRF 07的gp 41区来源于进化枝C病毒。为了比较CRF 07 gp 41与典型进化枝B病毒的差异，我们解析了CRF 07 gp 41核心区的晶体结构。与进化枝B gp 41相比，CRF 07 gp 41在螺旋束表面进化出更多的碱性和亲水性残基。基于序列比对，鉴定了位于HR 2七肽重复序列中间的超突变簇。这些残基的突变研究表明，这个网站是重要的艾滋病毒介导的细胞-细胞融合，并在病毒入侵过程中的构象变化起着关键作用。(C)2013 Elsevier Inc. All rights reserved.
HIV CRF07 B'/C is a strain circulating mainly in northwest region of China. The gp41 region of CRF07 is derived from a clade C virus. In order to compare the difference of CRF07 gp41 with that of typical clade B virus, we solved the crystal structure of the core region of CRF07 gp41. Compared with clade B gp41, CRF07 gp41 evolved more basic and hydrophilic residues on its helix bundle surface. Based on sequence alignment, a hyper-mutant cluster located in the middle of HR2 heptads repeat was identified. The mutational study of these residues revealed that this site is important in HIV mediated cell-cell fusion and plays critical roles in conformational changes during viral invasion. (C) 2013 Elsevier Inc. All rights reserved.