Quantitative T cell assay reflects infectious load of Mycobacterium tuberculosis in an endemic case contact model
Quantitative T cell assay reflects infectious load of Mycobacterium tuberculosis in an endemic case contact model
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DOI：
10.1086/427030
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发表时间：
2005-01-15
影响因子：
11.8
通讯作者：
Brookes, RH
中科院分区：
文献类型：
--
作者：
Hill, PC;Fox, A;Brookes, RH
背景资料。目前，用于评估新的结核病干预措施的可靠疗效标记物仅限于疾病和死亡。更准确地测量人类对结核分枝杆菌感染的免疫反应可能是重要的。早期分泌性抗原靶标6(ESAT-6)和培养滤液蛋白10(CFP-10)的定性酶联免疫斑点试验(ELISPOT)结果在检测结核分枝杆菌感染方面比纯化蛋白衍生物(PPD)皮试反应具有更高的特异性。我们评估了ELISPOT和PPD皮试对近期结核分枝杆菌暴露的定量反应。我们研究了定量PPD皮肤试验和PPD ELISPOT结果，根据对结核杆菌特异性抗原(ESAT-6/CFP-10)的体外干扰素伽马ELISPOT阳性或阴性，对1052名指标患者的健康家庭接触者进行了PPD皮肤试验和PPD ELISPOT结果的研究。然后，我们研究了定量PPD皮肤试验和PPD ELISPOT结果，这些患者接触的患者ESAT-6/CFP-10结果为阳性，根据与结核病患者的睡眠距离，自然暴露梯度为阳性。在ESAT-6/CFP-10阳性的受试者中，PPD皮试和PPD ELISPOT的阳性结果数均显著高于其他组(P
Background. Currently, reliable efficacy markers for assessment of new interventions against tuberculosis (TB) are limited to disease and death. More precise measurement of the human immune response to Mycobacterium tuberculosis infection may be important. A qualitative enzyme-linked immunospot assay (ELISPOT) result for early secretory antigenic target 6 (ESAT-6) and culture filtrate protein 10 (CFP-10) offers improved specificity over the purified protein derivative (PPD) skin test reaction in the detection of M. tuberculosis infection. We evaluated the quantitative ELISPOT and PPD skin test responses to recent M. tuberculosis exposure.Methods. We studied quantitative PPD skin test and PPD ELISPOT results in 1052 healthy household contacts of index patients with cases of sputum smear-positive and culture-positive TB in The Gambia, according to a positive or negative ex vivo interferon gamma ELISPOT response to M. tuberculosis-specific antigens (ESAT-6/CFP-10). We then studied the quantitative PPD skin test and PPD ELISPOT results in patient contacts who had positive ESAT-6/CFP-10 results against a natural exposure gradient according to sleeping proximity to a patient with TB.Results. The number of positive results was significantly greater for both PPD skin test and PPD ELISPOT in ESAT-6/CFP-10-positive subjects, compared with others (P