Internalisation of desmosomes and their entry into the endocytic pathway via late endosomes in MDCK cells. Possible mechanisms for the modulation of cell adhesion by desmosomes during development.
Internalisation of desmosomes and their entry into the endocytic pathway via late endosomes in MDCK cells. Possible mechanisms for the modulation of cell adhesion by desmosomes during development.
复制标题
MDCK 细胞中桥粒的内化及其通过晚期内体进入内吞途径。
作者：
I. Burdett
生长在正常Ca2+水平（约2mm）培养基中的MDCK细胞含有内化的桥粒，称为桥粒相关液泡（DAVs）。dav由保留在周围空泡膜平面上的一到三个斑块组成，它们进入内吞途径的情况已通过HRP、离子化铁蛋白和BSA/金结合电镜和免疫金标记的冷冻切片进行了研究。从根尖和基底侧表面提供给滤过生长的MDCK细胞的内噬示踪剂在一个共同的核周室中聚集，但在短（5-30分钟）的标记脉冲期间（无论是根尖还是基底侧），dav都没有被标记。只有当示踪剂从基底外侧表面取下，然后追踪2-18小时，才会标记dav。有人提出，内吞示踪剂通过桥粒空泡与晚期内体的关联进入dav。对桥粒成分（Dsg、DPI/II）、HRP和不依赖于离子的甘露糖6-磷酸受体（MPR）的抗体进行免疫标记研究，证实Dsg和DPI/II位于dav和晚期核内体中，但不在早期核内体中。在MPR-结构（溶酶体）中检测到Dsg的传代，但在较小程度上检测到DPI/II。在小鼠肾脏等发育组织中也观察到类似dav的结构。这种吞噬可能为处理不溶性连接蛋白提供了一种通用机制，特别是在细胞-细胞粘附模式中发生快速形态发生变化的情况下。
MDCK cells grown in media with normal levels of Ca2+ (approximately 2 mM) contain internalised desmosomes, referred to as desmosome-associated vacuoles (DAVs). The DAVs consist of one to three plaques retained in the plane of a surrounding vacuolar membrane, and their entry into the endocytic pathway has been investigated using HRP, cationized ferritin and BSA/gold in combination with electron microscopy and immunogold labelling of frozen sections. Endocytic tracers supplied from the apical and basolateral surfaces to filter-grown MDCK cells met in a common perinuclear compartment but DAVs were not labelled during short (5-30 minutes) pulses of marker, whether applied apically or basolaterally. Only when the tracers were taken up from the basolateral surface and then chased for periods of 2-18 hours, were DAVs labelled. It is proposed that entry of an endocytic tracer to DAVs occurs by the association of the desmosomal vacuole with late endosomes. Immunolabelling studies with antibodies to desmosomal components (to Dsg, DPI/II), to HRP and to the cation-independent mannose 6-phosphate receptor (MPR), confirmed that Dsg and DPI/II are located within DAVs and late endosomes, but not in early endosomes. Passage of Dsg, but to a lesser extent DPI/II, was detected in MPR- structures (lysosomes). DAV-like structures have also been observed in developing tissues such as mouse kidney. Such engulfment may provide a general mechanism for handling insoluble junctional proteins, particularly where rapid morphogenetic changes are occurring in the pattern of cell-cell adhesion.
DOI：
10.1016/s0021-9258(19)39844-8
发表时间：
1990-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. J. Green;D A Parry;P M Steinert;M. L. Virata;R. M. Wagner;B. Angst;L. A. Nilles
通讯作者：
K. J. Green;D A Parry;P M Steinert;M. L. Virata;R. M. Wagner;B. Angst;L. A. Nilles
影响因子：
3.5
作者：
Fuchs, P;Zörer, M;Wiche, G
通讯作者：
Wiche, G
影响因子：
3.2
作者：
BIRRELL, GB;HEDBERG, KK;GRIFFITH, OH
通讯作者：
GRIFFITH, OH