Effects of Different Host Plants on the Growth, Reproduction and Physiological Enzyme Activity of Dendrolimus houi Lajonquiere (Lepidoptera: Lasiocampidae)
Effects of Different Host Plants on the Growth, Reproduction and Physiological Enzyme Activity of Dendrolimus houi Lajonquiere (Lepidoptera: Lasiocampidae)
复制标题
DOI：
10.13275/j.cnki.lykxyj.2022.005.007
复制
发表时间：
2022-10-01
期刊：
影响因子：
--
通讯作者：
Liang Guang-hong
中科院分区：
文献类型：
--
作者：
Shen Hai-yan;Liang Yun;Liang Guang-hong
[目的]根据不同寄主植物对华山松毛虫生长、繁殖、解毒酶和消化酶活性的影响，分析华山松毛虫对不同寄主植物的生理适应机制，为开展新型混交林生态调控提供依据。[方法]室内饲养柳杉、侧柏幼虫和成虫，测定幼虫、蛹的发育历期和成虫的繁殖力。Franco、柏木、云南松(Pinus云南松)和马尾松Pinus Massoniana Lamb.测定了摄食不同寄主植物的日本血吸虫5龄幼虫的解毒酶(谷胱甘肽S转移酶、细胞色素P450和羧酸酯酶)和消化酶(蛋白酶、淀粉酶和脂肪酶)的活性。[结果]取食云杉、云杉、东方云杉和云南云杉的厚朴幼虫具有完整的生活史，而取食马尾松的幼虫仅存活至5龄，没有完整的生活史。同时，与其他寄主植物相比，取食云杉和粉纹夜蛾幼虫和蛹的发育历期较短，雌蛹较重，成虫产卵量较大。摄食云南云杉、侧柏和马尾松5龄幼虫的GSTs和CYP450活性显著高于取食云南云杉、侧柏和马尾松的幼虫(P
[Objective] To provide insights into carrying out ecological regulation of new mixed forest, the physiological adaptation mechanism of Dendrolimus houi Lajonquiere to different host plants was analyzed based on the effects of different host plants on the growth, reproduction, detoxification enzyme and digestive enzyme activities of D. houi. [Methods] The developmental duration of the larvae and pupae as well as the fecundity of adult of D. houi were measured through indoor rearing in hots plants of Cryptomeria fortunei Hooibrenk, Platycladus orientalis (L.) Franco, Cupressus funebris Endl., Pinus yunnanensis Franch. and Pinus massoniana Lamb. The activities of detoxification enzymes (i. e. glutathione S-transferases (GSTs), cytochrome P450 (CYP450), and carboxylesterase (CarE)) and digestive enzymes (i. e. protease, amylase (AMS), and lipase (LPS)) in the 5th instar larvae of D. houi feeding on different host plants were also assayed. [Results] D. houi larvae feeding on C. fortunei, C. funebris, P. orientalis and P. yunnanensis had a complete life cycle, while the larvae feeding on P. massoniana only survived to the 5th instar and did not have a complete life cycle. At the same time, the larvae and pupae of D. houi feeding on C. fortunei and C. funebris had shorter developmental duration, heavier female pupae and more fecundity of adult compared with other host plants. The activities of GSTs and CYP450 in the 5th instar larvae of D. houi feeding on C. fortunei and C. funebris were significantly higher than those feeding on P. yunnanensis, P. orientalis and P. massoniana (P