Identification of Cancer-Associated Fibroblasts in Circulating Blood from Patients with Metastatic Breast Cancer
Identification of Cancer-Associated Fibroblasts in Circulating Blood from Patients with Metastatic Breast Cancer
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DOI：
10.1158/0008-5472.can-15-1633
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发表时间：
2015-11-15
期刊：
影响因子：
11.2
通讯作者：
El-Ashry, Dorraya
中科院分区：
文献类型：
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作者：
Ao, Zheng;Shah, Sanket H.;El-Ashry, Dorraya
肿瘤微环境中的肿瘤相关成纤维细胞(CAF)通过尚未阐明的机制促进肿瘤的转移。在这项研究中，我们使用了我们团队开发的基于大小的微滤技术来检查是否可以在转移性乳腺癌患者的外周血中区分FAP和α-SMA共表达(CCAF)识别的循环CAF。在一项乳腺癌患者的初步研究中，我们检测到30/34(88%)的转移性疾病患者(MET组)和3/13(23%)长期无病生存的局限性乳腺癌患者(LOC组)CCAFS的存在。在健康供者中没有检测到定义的CCAF。此外，与LOC组相比，MET组的CCAF和循环肿瘤细胞(CTC)都显著增加。因此，CCAF的存在与临床转移有关，提示CCAF可以补充CTC作为转移性乳腺癌的临床相关生物标志物。(C)2015年AACR。
Metastasis is facilitated by cancer-associated fibroblasts (CAF) in the tumor microenvironment through mechanisms yet to be elucidated. In this study, we used a size-based microfilter technology developed by our group to examine whether circulating CAF identified by FAP and alpha-SMA coexpression (cCAF) could be distinguished in the peripheral blood of patients with metastatic breast cancer. In a pilot study of patients with breast cancer, we detected the presence of cCAFs in 30/34 (88%) patients with metastatic disease (MET group) and in 3/13 (23%) patients with localized breast cancer (LOC group) with long-term disease-free survival. No cCAFs as defined were detected in healthy donors. Further, both cCAF and circulating tumor cells (CTC) were significantly greater in the MET group compared with the LOC group. Thus, the presence of cCAF was associated with clinical metastasis, suggesting that cCAF may complement CTC as a clinically relevant biomarker in metastatic breast cancer. (C) 2015 AACR.