Principles of using Cold Atmospheric Plasma Stimulated Media for Cancer Treatment.
Principles of using Cold Atmospheric Plasma Stimulated Media for Cancer Treatment.
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DOI：
10.1038/srep18339
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发表时间：
2015-12-17
影响因子：
4.6
通讯作者：
Keidar M
中科院分区：
文献类型：
--
作者：
Yan D;Talbot A;Nourmohammadi N;Cheng X;Canady J;Sherman J;Keidar M
到目前为止，冷大气等离子体(CAP)在体外和小鼠模型中对数十种癌细胞株具有显著的抗癌能力。传统上，CAP直接应用于照射癌细胞或肿瘤组织。在过去的三年里，CAP照射的介质也被发现与直接的CAP治疗一样有效地杀死癌细胞。作为一种新的策略，使用CAP刺激(CAPS)介质已成为一种有前途的抗癌工具。在这项研究中，我们展示了优化CAPS培养基对胶质母细胞瘤细胞和乳腺癌细胞的抗癌能力的几个原则。具体地说，在多孔板上使用更大的孔，较小的等离子体源和介质之间的间隙，以及较小的介质体积，使我们能够在不增加治疗时间的情况下获得更强的抗癌帽介质组合物。此外，半胱氨酸是CAPS介质中有效活性物种的主要靶标。与乳腺癌细胞相比，胶质母细胞瘤细胞对CAPS介质的抵抗力更强。胶质母细胞瘤细胞比乳腺癌细胞消耗有效活性物质的速度更快。与一氧化氮相反，过氧化氢更有可能是有效的反应物种。
To date, the significant anti-cancer capacity of cold atmospheric plasma (CAP) on dozens of cancer cell lines has been demonstrated in vitro and in mice models. Conventionally, CAP was directly applied to irradiate cancer cells or tumor tissue. Over past three years, the CAP irradiated media was also found to kill cancer cells as effectively as the direct CAP treatment. As a novel strategy, using the CAP stimulated (CAPs) media has become a promising anti-cancer tool. In this study, we demonstrated several principles to optimize the anti-cancer capacity of the CAPs media on glioblastoma cells and breast cancer cells. Specifically, using larger wells on a multi-well plate, smaller gaps between the plasma source and the media, and smaller media volume enabled us to obtain a stronger anti-cancer CAPs media composition without increasing the treatment time. Furthermore, cysteine was the main target of effective reactive species in the CAPs media. Glioblastoma cells were more resistant to the CAPs media than breast cancer cells. Glioblastoma cells consumed the effective reactive species faster than breast cancer cells did. In contrast to nitric oxide, hydrogen peroxide was more likely to be the effective reactive species.