Beads on the run: beads as alternative tools for chemotaxis assays.
Beads on the run: beads as alternative tools for chemotaxis assays.
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DOI：
10.1007/978-1-61779-207-6_30
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发表时间：
2011
影响因子：
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通讯作者：
Eric Théveneau;R. Mayor
中科院分区：
文献类型：
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作者：
Eric Théveneau;R. Mayor
细胞迁移是从细菌寻找食物到纠正神经元网络模式的各种过程所必需的。感知外部线索的能力对于细胞获得方向和实现目标至关重要。到目前为止，对趋化性的研究主要集中在单个细胞上，因此现有的工具被设计成在单个细胞水平上监测细胞的行为。然而，随着集体细胞迁移现在被广泛接受为细胞从发育到癌症迁移的主要方式，如何实现趋化也必须在更大的范围内提出问题。在这里，我们提出了两种适合于单细胞、细胞片和细胞外植体的趋化分析方法。使用肝素涂层珠子和高真空硅脂的简单组合，这些技术可以适应各种培养条件。它们允许延时研究、高分辨率显微镜，并且可以在不额外成本的情况下设置。
Cell migration is required for a wide variety of processes from bacteria seeking for food to correct patterning of neuronal networks. The ability to sense external cues is critical for cells to get directions and reach their goals. So far, studies on chemotaxis have mainly focused their attention on individual cells and therefore available tools are designed to monitor cell behavior at the single cell level. However, as collective cell migration is now widely accepted as a main mode of cell migration from development to cancer, the question of how chemotaxis is achieved has also to be asked on a bigger scale. Here, we present two chemotaxis assays suitable for single cells, cell sheets, and cell explants. Using a simple combination of heparin-coated beads and high vacuum silicone grease, these techniques can be adapted to a wide variety of culture conditions. They allow time-lapse study, high-resolution microscopy, and can be set up at no extra cost.