Structural basis of eukaryotic gene transcription
Structural basis of eukaryotic gene transcription
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DOI：
10.1016/j.febslet.2004.11.027
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发表时间：
2005-02-07
期刊：
影响因子：
3.5
通讯作者：
Kornberg, RD
中科院分区：
文献类型：
--
作者：
Boeger, H;Bushnell, DA;Kornberg, RD
RNA聚合酶II启动子已被分离在转录激活和抑制状态。拓扑学和核酸酶消化分析揭示了转录激活后核小体去除和重组之间的动态平衡。并进一步表明，核小体是通过驱逐历史八聚体而不是通过滑动来去除的。启动子一旦暴露，就与RNA聚合酶II、一般转录因子和介体组装成类似于3 MDa的转录起始复合物。X射线晶体学已经揭示了RNA聚合酶II在转录过程中的原子分辨率结构。这种分析的扩展已经显示了核苷酸如何经历选择、聚合和最终从转录复合物中释放。X射线和电子晶体学已经导致了整个转录起始复合物的图片，阐明了启动子识别，DNA解旋，流产启动和启动子逃逸的机制。(C)2004年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
An RNA polymerase II promoter has been isolated in transcriptionally activated and repressed states. Topological and nuclease digestion analyses have revealed a dynamic equilibrium between nucleosome removal and reassembly upon transcriptional activation. and have further shown that nucleosomes are removed by eviction of historic octamers rather than by sliding. The promoter, once exposed, assembles with RNA polymerase II, general transcription factors, and Mediator in a similar to3 MDa transcription initiation complex. X-ray crystallography has revealed the structure of RNA polymerase II, in the act of transcription, at atomic resolution. Extension of this analysis has shown how nucleotides undergo selection, polymerization, and eventual release from the transcribing complex. X-ray and electron crystallography have led to a picture of the entire transcription initiation complex, elucidating the mechanisms of promoter recognition, DNA unwinding, abortive initiation, and promoter escape. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.