Featured Species-Specific Loops Are Found in the Crystal Structure of Mhp Eno, a Cell Surface Adhesin From Mycoplasma hyopneumoniae
Featured Species-Specific Loops Are Found in the Crystal Structure of Mhp Eno, a Cell Surface Adhesin From Mycoplasma hyopneumoniae
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在 Mhp Eno 的晶体结构中发现了特征性的物种特异性环，Mhp Eno 是一种来自猪肺炎支原体的细胞表面粘附素
DOI：
10.3389/fcimb.2019.00209
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发表时间：
2019-06-13
影响因子：
5.7
通讯作者：
Shao, Guoqing
中科院分区：
文献类型：
--
作者：
Chen, Rong;Yu, Yanfei;Shao, Guoqing
烯醇化酶是一种进化上保守的酶，参与糖酵解和糖异生过程。猪肺炎支原体（Mycoplasma hyopteriasis）属于支原体属（Mycoplasma），其种类是无壁的并且是最小的自我复制细菌之一，并且是全球养猪业中重要的定殖呼吸道病原体。猪肺炎支原体烯醇化酶（Mhp Eno）的表达在感染后显著增加，并且先前被发现是毒力因子候选者。我们的研究表明，Mhp Eno是一种细胞表面定位的蛋白，可以粘附到猪气管上皮细胞（STECs）。Mhp Eno抗体可特异性抑制对STEC的粘附。Mhp Eno能与纤溶酶原发生高亲和力的识别和相互作用。在这里，第一晶体结构的支原体烯醇化酶从支原体猪肺炎被确定。该结构在S3/H1、H6/S6、H7/H8和H13区域显示出Mhp Eno的独特特征。所有这些区域都比其他烯醇化酶长，并且暴露在Mhp Eno表面上，使它们能够接近宿主分子。这些结果表明，Mhp Eno具有特定的结构特征，并作为一个多功能的粘附素对支原体细胞表面。
Enolase is an evolutionarily conserved enzyme involved in the processes of glycolysis and gluconeogenesis. Mycoplasma hyopneumoniae belongs to Mycoplasma, whose species are wall-less and among the smallest self-replicating bacteria, and is an important colonizing respiratory pathogen in the pig industry worldwide. Mycoplasma hyopneumoniae enolase (Mhp Eno) expression is significantly increased after infection and was previously found to be a virulence factor candidate. Our studies show that Mhp Eno is a cell surface-localized protein that can adhere to swine tracheal epithelial cells (STECs). Adhesion to STECs can be specifically inhibited by an Mhp Eno antibody. Mhp Eno can recognize and interact with plasminogen with high affinity. Here, the first crystal structure of the mycoplasmal enolase from Mycoplasma hyopneumoniae was determined. The structure showed unique features of Mhp Eno in the S3/H1, H6/S6, H7/H8, and H13 regions. All of these regions were longer than those of other enolases and were exposed on the Mhp Eno surface, making them accessible to host molecules. These results show that Mhp Eno has specific structural characteristics and acts as a multifunctional adhesin on the Mycoplasma hyopneumoniae cell surface.