Markers of activity in clinically recovered human leukocyte antigen-DR17-positive sarcoidosis patients
Markers of activity in clinically recovered human leukocyte antigen-DR17-positive sarcoidosis patients
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DOI：
10.1183/09031936.03.00059103
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发表时间：
2003-01-01
影响因子：
24.3
通讯作者：
Grunewald, J
中科院分区：
文献类型：
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作者：
Planck, A;Eklund, A;Grunewald, J
斯堪的纳维亚人类白细胞抗原 DR17 阳性（DR17+）结节病患者的特点是预后良好。他们还揭示了疾病发作时表达 T 细胞受体 V 基因片段 AV2S3 的 T 淋巴细胞在支气管肺泡灌洗液中积聚。本研究的作者希望确定 AV2S3 T 淋巴细胞积聚从疾病发作到临床缓解的过程中是否发生变化，以及这与其他活动参数有何关系。在疾病发作时以及疾病的临床和影像学体征自发消退后，对 9 名 DR17+ 结节病患者的支气管肺泡灌洗液和血清进行了检查。在临床恢复后，对 9 名肺积聚 CD4+ AV2S3+ T 细胞的 DR17+ 患者进行了研究。复查时，支气管肺泡灌洗液中 CD4+ AV2S3+ 淋巴细胞的百分比标准化（29% vs 5.4%）。还观察到淋巴细胞百分比显着减少（14% vs 4.4%）、细胞浓度降低（179 x 10(6.)L(-1) vs 111x10(6.)L-(1)）和 CD4/CD8 比率（5.2 vs 2.4）。恢复后血清中血管紧张素转换酶活性（24.9 vs 14.0 U(.)mL(-1)）和新蝶呤水平（7.8 vs 5.3 nmol(.)L(-1)）显着下降。这些结果表明，支气管肺泡灌洗液中局部积累的AV2S3阳性T淋巴细胞参与了该组结节病的发病过程。
Scandinavian human leukocyte antigen-DR17-positive (DR17+) sarcoidosis patients are characterised by a good prognosis. They also reveal an accumulation in bronchoalveolar lavage fluid of T-lymphocytes expressing the T-cell receptor V gene segment AV2S3 at disease onset. The authors of this study wished to establish whether AV2S3 T-lymphocyte accumulation changes from disease onset to clinically resolved disease and how this relates to other activity parameters.Bronchoalveolar lavage fluid and serum from nine DR17+ sarcoidosis patients were examined at disease onset and after spontaneous resolution of clinical and radio-graphical signs of disease.Nine DR17+ patients with lung accumulated CD4+ AV2S3+ T-cells were investigated after clinically recovery. At re-examination the percentage of CD4+ AV2S3+ lymphocytes in bronchoalveolar lavage fluid was normalised (29 versus 5.4%). A significant reduction in lymphocyte percentage (14 versus 4.4%,) and a decrease in cellular concentration (179 x 10(6.)L(-1) versus 111x10(6.)L-(1)) and CD4/CD8 ratio (5.2 versus 2.4) were also seen. In serum, the activity of angiotensin-converting enzyme (24.9 versus 14.0 U(.)mL(-1)) as well as the levels of neopterin (7.8 versus 5.3 nmol(.)L(-1)) decreased significantly after recovery.These results indicate that the locally accumulated AV2S3 positive T-lymphocytes in bronchoalveolar lavage are involved in the pathogenic process of sarcoidosis in this patient group.