GROWTH-FACTORS IN WOUND-HEALING - SINGLE AND SYNERGISTIC EFFECTS ON PARTIAL THICKNESS PORCINE SKIN WOUNDS
GROWTH-FACTORS IN WOUND-HEALING - SINGLE AND SYNERGISTIC EFFECTS ON PARTIAL THICKNESS PORCINE SKIN WOUNDS
复制标题
DOI：
10.1172/jci114210
复制
发表时间：
1989-08-01
影响因子：
15.9
通讯作者：
ANTONIADES, HN
中科院分区：
文献类型：
--
作者：
LYNCH, SE;COLVIN, RB;ANTONIADES, HN
几种生长因子可能是伤口愈合的介质，尽管它们的实际作用、相互作用和治疗用途尚未确定。我们选择了六种具有良好特征的人生长因子，通过局部应用于猪的标准化皮肤伤口进行了详细的研究：表皮生长因子（EGF），转化生长因子。和度量。(TGF -.alpha。和TGF- β)、成纤维细胞生长因子（FDF）、胰岛素样生长因子- i （IGF-I）和血小板衍生生长因子（PDGF）。当单次剂量达到1500 ng时，只有TGF-。产生了显著的组织反应，如新结缔组织体积的增加，胶原蛋白含量和成熟度的增加，以及血管生成的增加。然而,TGF度量。炎症反应增强，上皮细胞分化异常，上皮细胞体积减小，添加igf - 1可逆转这一过程。重组PDGF-2同二聚体，如果与重组igf - 1联合使用，可引起新结缔组织体积、胶原含量和成熟度的类似增加，但没有增加炎症。此外，这种结合刺激了正常分化的上皮细胞数量的增加。PDGF-2和igf - 1的协同作用以2:1的重量比为最佳。在测试的6个单独因素和9个组合中，PDGF-2和IGF-I或PDGF-2和TGF- α的组合。在没有增加炎症的情况下是最有效的愈合刺激物。
Several growth factors are potentially mediators of wound healing, although their actual roles, interactions, and therapeutic use are not established. Six well-characterized human growth factors were chosen for detailed investigation by topical application to standardized skin wounds in swine: epidermal growth factor (EGF), transforming growth factors .alpha. and .beta. (TGF-.alpha. and TGF-.beta.), fibroblast growth factor (FDF), insulin-like growth-factor-I (IGF-I), and platelet-derived growth factor (PDGF). When applied singly in doses up to 1,500 ng, only TGF-.beta. produced a marked tissue response, as demonstrated by an increase in the new connective tissue volume, the collagen content and maturity, and increased angiogenesis. However, TGF-.beta. enhanced inflammation and caused abnormal epithelial differentiation and decreased epithelial volume, the last reversed by addition of IGF-I. Recombinant PDGF-2 homodimer, if given in combination with recombinant IGF-I, caused a similar increase in the new connective tissue volume and collagen content and maturity, but without increased inflammation. In addition, this combination stimulated increased amounts of epithelium with normal differentiation. The synergy of PDGF-2 and IGF-I was optimal at a ratio of 2:1 by weight. Of the six individual factors and nine combinations tested, the combinations of PDGF-2 and IGF-I or PDGF-2 and TGF-.alpha. were the most potent stimulators of healing in the absence of increased inflammation.