PRMT5 is necessary to form distinct cartilage identities in the knee and long bone.
PRMT5 is necessary to form distinct cartilage identities in the knee and long bone.
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DOI：
10.1016/j.ydbio.2019.08.012
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发表时间：
2019-12
影响因子：
2.7
通讯作者：
Janani Ramachandran;Zhaoyang Liu;R. Gray;S. Vokes
中科院分区：
文献类型：
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作者：
Janani Ramachandran;Zhaoyang Liu;R. Gray;S. Vokes
在骨骼发育过程中，肢体祖细胞被指定为软骨细胞，随后分化成专门的软骨隔室。我们以前表明，精氨酸二甲基转移酶，PRMT5，是必不可少的调节规范的祖细胞成软骨细胞内早期肢芽。在这里，我们报告说，PRMT5调节一个单独的祖域，产生髌骨的生存。与其在膝盖发育中的作用无关，PRMT 5调节长骨内几种不同类型的软骨细胞分化。缺乏PRMT5的软骨细胞在肥大软骨细胞分化中具有显著的阻断作用，并且以异常基因表达为标志。PRMT5在成人阶段对关节软骨和肥大细胞的身份仍然很重要，表明在这些组织的稳态中发挥着持续的作用。我们的结论是，PRMT5是所需的早期和晚期软骨形成专业化的不同步骤，因此是长骨发育和维护的多个方面的关键组成部分。
During skeletal development, limb progenitors become specified as chondrocytes and subsequently differentiate into specialized cartilage compartments. We previously showed that the arginine dimethyl transferase, PRMT5, is essential for regulating the specification of progenitor cells into chondrocytes within early limb buds. Here, we report that PRMT5 regulates the survival of a separate progenitor domain that gives rise to the patella. Independent of its role in knee development, PRMT5 regulates several distinct types of chondrocyte differentiation within the long bones. Chondrocytes lacking PRMT5 have a striking blockage in hypertrophic chondrocyte differentiation and are marked by abnormal gene expression. PRMT5 remains important for articular cartilage and hypertrophic cell identity during adult stages, indicating an ongoing role in homeostasis of these tissues. We conclude that PRMT5 is required for distinct steps of early and late chondrogenic specialization and is thus a critical component of multiple aspects of long bone development and maintenance.