In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae.
In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae.
复制标题
肺炎克雷伯菌固氮调节操纵子 nifLA 的体外转录。
DOI：
10.1128/jb.169.6.2876-2880.1987
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发表时间：
1987
影响因子：
3.2
通讯作者：
Kustu,S
中科院分区：
文献类型：
--
作者：
Wong,PK;Popham,D;Keener,J;Kustu,S
In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by covalent modification (A.J. Ninfa and B. Magasanik, Proc. Natl. Acad. Sci. USA 83:5909, 1986). Two DNA-binding sites for NTRC lie approximately 150 base pairs upstream of the nifLA promoter.
