Simple sequence repeat-based consensus linkage map of Bombyx mori
Simple sequence repeat-based consensus linkage map of Bombyx mori
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DOI：
10.1073/pnas.0507794102
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发表时间：
2005-11-08
影响因子：
11.1
通讯作者：
Huang, YP
中科院分区：
文献类型：
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作者：
Miao, XX;Xu, SJ;Huang, YP
作为国际基因组学计划的一部分，我们利用518个简单序列重复（SSR，或微卫星）标记建立了家蚕（家蚕）的遗传连锁图谱，家蚕是经济和文化上重要的鳞翅目昆虫。利用2500个（CA）nand (CT)n SSR标记对6个有代表性的家蚕品系进行了调查，结果显示多态性为17-24%，表明长期近交导致了高度的纯合性。通过对一株F雄性与一株C108雌性杂交的189个回交后代的基因分型，在性状较好的大枣和C108品系中建立29个SSR连锁群。通过对F，雌性与C108雄性交配的22个回交后代进行基因分型，进一步将聚类集中到28个群体。利用先前定位的基因、cDNA序列和克隆的随机扩增的多态性dna开发的可见突变和扩增的多态性序列标记，进一步将这组SSR连锁群分配到家蚕的28条染色体（已建立的连锁群）上。通过将一个可见突变p （plain，幼虫标记）和29个保守性较好的昆虫基因整合到基于ssr的家蚕遗传图谱中，构建了第二代共识家蚕遗传图谱，每个连锁群包含7-40个标记，总图谱长度约为3431.9 cM，证明了该图谱的高效基因分型和在鳞翅目及其他昆虫的协同研究中具有潜在的应用价值。
We established a genetic linkage map employing 518 simple sequence repeat (SSR, or microsatellite) markers for Bombyx mori (silkworm), the economically and culturally important lepidopteran insect, as part of an international genomics program. A survey of six representative silkworm strains using 2,500 (CA)nand (CT)n-based SSR markers revealed 17-24% polymorphism, indicating a high degree of homozygosity resulting from a long history of inbreeding. Twenty-nine SSR linkage groups were established in well characterized Dazao and C108 strains based on genotyping of 189 backcross progeny derived from an F, male mated with a C108 female. The clustering was further focused to 28 groups by genotyping 22 backcross progeny derived from an F, female mated with a C108 male. This set of SSR linkage groups was further assigned to the 28 chromosomes (established linkage groups) of silkworm aided by visible mutations and cleaved amplified polymorphic sequence markers developed from previously mapped genes, cDNA sequences, and cloned random amplified polymorphic DNAs. By integrating a visible mutation p (plain, larval marking) and 29 well conserved genes of insects onto this SSR-based linkage map, a second generation consensus silkworm genetic map with a range of 7-40 markers per linkage group and a total map length of approximate to 3431.9 cM was constructed and its high efficiency for genotyping and potential application for synteny studies of Lepidoptera and other insects was demonstrated.