ISOLATION OF RAT LIVER PLASMA MEMBRANES
ISOLATION OF RAT LIVER PLASMA MEMBRANES
复制标题
大鼠肝血浆膜的分离
DOI：
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发表时间：
1970
影响因子：
7.8
通讯作者：
H. Hendrickson
中科院分区：
文献类型：
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作者：
O. Touster;N. Aronson;J. Dulaney;H. Hendrickson
大鼠肝脏的核苷酸焦磷酸酶和磷酸二酯酶I已被发现主要定位于细胞颗粒中，相对于最普遍接受的质膜标记5‘-核苷酸酶高度富含，因此它们本身应该被指定为质膜定位。利用观察到的质膜在含有核级和微体级分的等渗蔗糖中沉积的现象，从每个级分获得质膜制剂。这两种制剂在化学成分和酶组成上相似。此外，本研究开发的制备方法似乎给出了得率、纯度和重现性的最佳组合。考虑了核苷酸焦磷酸酶和磷酸二酯酶I的可能同一性的问题，并提出了证据表明，这些活性可能是同一种酶的表现。
Nucleotide pyrophosphatase and phosphodiesterase I of rat liver have been found to be localized primarily in cell particulates highly enriched with respect to the most commonly accepted plasma membrane marker, 5'-nucleotidase, and therefore should themselves be assigned a plasma membrane localization. The observation that plasma membranes sediment in isotonic sucrose with both nuclear and microsomal fractions was exploited to obtain plasma membrane preparations from each fraction. Both preparations are similar in chemical and enzymic composition. Moreover, the preparative method developed in this study appears to give the best combination of yield, purity, and reproducibility available. The question of the possible identity of nucleotide pyrophosphatase and phosphodiesterase I is considered, and evidence is presented suggesting that these activities may be manifestations of the same enzyme.