Altered immune function of Octodonta nipae (Maulik) to its pupal endoparasitoid, Tetrastichus brontispae Ferriere
Altered immune function of Octodonta nipae (Maulik) to its pupal endoparasitoid, Tetrastichus brontispae Ferriere
复制标题
改变了 Octodonta nipae (Maulik) 对其蛹内寄生蜂 Tetrastichus brontispae Ferriere 的免疫功能
DOI：
10.1016/j.cbpb.2016.04.001
复制
发表时间：
2016-08-01
影响因子：
2.2
通讯作者：
Yu, Xiao-Qiang
中科院分区：
文献类型：
--
作者：
Meng, E.;Tang, Baozhen;Yu, Xiao-Qiang
大多数关于内寄生蜂改变免疫反应的研究仅限于姬蜂总科及其宿主之间的相互作用，而除了一些黄蜂（例如翼手蜂科）外，很少描述小蜂总科寄生对宿主的影响。内寄生蜂Tetrastastichus brontispae Ferriere属于膜翅目甲虫，对于控制一些鞘翅目甲虫（例如中国南方的入侵物种Octodonta nipae）具有巨大潜力。然而，O. nipae 蛹逃离黑色素封装的生理机制尚未得到证实。在本研究中，研究了寄生对其蛹宿主 O. nipae 免疫功能的影响。粒细胞和浆细胞在2~48 h内均可吞噬细菌,粒细胞、浆细胞和类胞浆细胞均为酚氧化酶/酚氧化酶阳性血细胞,表明粒细胞、浆细胞和类胞浆细胞是云斑潜蝇蛹中主要的免疫活性血细胞。 T. brontispae寄生导致96小时血细胞活力和扩散百分比显着增加，24小时粒细胞百分比增加，但对总血细胞计数没有影响，仅在12和72小时酚氧化酶活性增强，而寄生后96小时血淋巴黑化时间显着延长。这些结果表明，全身活性和局部活性调节的混合物被用于Tbrontispae以逃避宿主在O. nipae蛹中的封装。本研究有助于了解寄生蜂使用的毒力策略的多样性。 (C) 2016 爱思唯尔公司保留所有权利。
Most studies on the contribution of the altered immune response by endoparasitoid have been restricted to the interactions between Ichneumonoidea and their hosts, while effects of parasitism by Chalcidoidea on the hosts have rarely been characterized except some wasps such as Pteromalidae. Endoparasitoid Tetrastichus brontispae Ferriere, belonging to Eulophidae (Hymenoptera), has a great potential to control some Coleopteran beetles such as Octodonta nipae, one invasive species in southern China. However, the physiological mechanism underlying the escape from the melanotic encapsulation in O. nipae pupae has not been demonstrated. In the present study, effects of parasitism on the immune function of its pupal host O. nipae were investigated. The combining results that granulocytes and plasmatocytes could phagocytize bacteria from 2 to 48 h and granulocytes, plasmatocytes and oenocytoids were prophenoloxidase/phenoloxidase positive hemocytes indicated that granulocytes, plasmatocytes and oenocytoids were the main immunocompetent hemocytes in O. nipae pupae. Parasitism by T. brontispae resulted in a significant increase in the percentage of hemocytes viability and spreading at 96 h, growing percentage of granulocytes at 24 h but no effects on the total hemocyte counts, and an enhanced phenoloxidase activity only at 12 and 72 h while a significantly longer melanization time of the hemolymph at 96 h following parasitism. These results indicate that mixtures of systemic active and local active regulation are used for T brontispae to escape host encapsulation in O. nipae pupae. The present study contributes to the understanding of the diversity of virulence strategies used by parasitoids. (C) 2016 Elsevier Inc All rights reserved.